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> Human ApoE ELISA Kit

Human ApoE ELISA Kit

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Référence ELH-ApoE-1

Conditionnement : 1PlateKit

Marque : RayBiotech


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Human ApoE ELISA KitHuman ApoE ELISA KitHuman ApoE ELISA KitHuman ApoE ELISA KitHuman ApoE ELISA KitHuman ApoE ELISA Kit5 Plate Kit Packaging (one box)

Human ApoE ELISA Kit

Human ApoE ELISA Kit

RayBio® Human ApoE ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

Contact Us for Bulk Request

Product Description

Specifications

Size1 Plate Kit, 2 Plate Kit, 5 Plate Kit
SpeciesHuman
Sensitivity
1.5 ng/ml

Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology.

Detection Range
1.5 ng/ml - 400 ng/ml
Specificity
This ELISA antibody pair detects human ApoE (isoforms ApoE2, ApoE3 and ApoE4).
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum
Recommended Dilution (Serum/Plasma)
1,000 fold
Accession Number
P02649
Gene Id
348
Gene Symbols
APOE
Protein Name / Synonyms
Apolipoprotein E (Apo-E)
Quantitative/Semi-QuantitativeQuantitative
Solid Support
96-well Microplate
Design PrincipleSandwich-based
Method Of DetectionColorimetric
Research AreaInflammation
Shipping TypeBlue ice
Storage≤-20°C

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Customize This Human ApoE ELISA

We manufacture our ELISAs in-house, so we can build a custom version for your study.

  • Post-translational modifications (PTMs): Detect modified Apolipoprotein E (ApoE), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
  • Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
  • Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist

Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.

Learn more about custom ELISA development

documents

Datasheet (ELH-ApoE.pdf)
SDS (ELH-ApoE.pdf)
Excel (Sandwich-ELISA-Analysis-Tool.xlsm)

citations

  • Neutralization of pathogenic PC-OxPL by AAV-delivered scFv as a therapeutic strategy for amyotrophic lateral sclerosis

    Species:
    Human
    Sample Type:
    Cerebrospinal Fluid (ALS patients vs healthy controls); Plasma
    Year:
    2026
    Journal Name:
    Molecular therapy. Advances
    PubMed ID:
    42614391

  • Correlation of APOE polymorphism, expression, and plasma levels with cardiac comorbidities among lipodystrophy in HIV-infected patients

    Year:
    2025
    Journal Name:
    Clinica Chimica Acta
    PubMed ID:
    39313065

  • Serological profiling reveals hsa-miR-451a as a possible biomarker of anaphylaxis

    Year:
    2022
    Journal Name:
    JCI insight
    PubMed ID:
    35202004

  • Biomarker Discovery for Early Diagnosis of Papillary Thyroid Carcinoma Using High-Throughput Enhanced Quantitative Plasma Proteomics

    Species:
    Human
    Sample Type:
    Serum
    Year:
    2023
    Journal Name:
    Journal of Proteome Research

  • Association of lipoproteins and thyroid hormones with cognitive dysfunction in patients with systemic lupus erythematosus

    Species:
    Human
    Year:
    2021
    Journal Name:
    BMC Rheumatology
    PubMed ID:
    34103098

  • Elevated levels of apolipoprotein A4 in umbilical cord serum from the maternal major depressive disorder

    Year:
    2024
    Journal Name:
    J Obstet Gynaecol Res
    PubMed ID:
    39319776

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