RayBio® Human bFGF ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
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Human bFGF ELISA Kit
Human bFGF ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 50 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 50 pg/ml - 10000 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | P09038 |
| Gene Id | 2247 |
| Gene Symbols | FGF2, FGFB |
| Protein Name / Synonyms | Fibroblast growth factor 2 (FGF-2) (Basic fibroblast growth factor) (bFGF) (Heparin-binding growth factor 2) (HBGF-2) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
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Target Background
Fibroblast growth factor-2 (FGF-2), also referred to as basic FGF, is a member of a family of growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuroectodermal origin. Additional members of the FGF family include the oncogenes FGF-3 (Int2) and FGF-4 (HST/Kaposi), FGF-5, FGF-6, FGF-7 (KGF), FGF-8 (AIGF), FGF-9 (GAF) and FGF-10. Members of the FGF family share 30-55% amino acid sequence identity and similar gene structure, and are capable of transforming cultured cells when overexpressed in transfected cells. Cellular receptors for FGFs are members of a second multigene family including four tyrosine kinases, designated Flg (FGFR-1), Bek (FGFR-L), TKF and FGFR-3
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human bFGF ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified bFGF (FGF-2 / FGF2), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
Excel (Sandwich-ELISA-Analysis-Tool.xlsm)
citations
Glycosaminoglycan-Inspired Polymer Brushes Promote Human Mesenchymal Stem Cell Proliferation While Retaining Trilineage Differentiation Potential
- Species:
- Human
- Sample Type:
- Other / Unspecified (bFGF solution, polymer brush-bound bFGF)
- Year:
- 2026
- Journal Name:
- ACS Applied Materials & Interfaces
- PubMed ID:
- 41521470
Promotion of right ventricular outflow tract reconstruction using a novel cardiac patch incorporated with hypoxia-pretreated urine-derived stem cells
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (supernatant from cultured cell-scaffold complex)
- Year:
- 2022
- Journal Name:
- Bioactive Materials
- PubMed ID:
- 35310356
Braided suture-reinforced fibrous yarn bundles as a scaffold for tendon tissue engineering in extensor digitorum tendon repair
- Species:
- Rabbit
- Sample Type:
- rabbit tendon-derived fibroblasts (rTFs)
- Year:
- 2022
- Journal Name:
- Chemical Engineering Journal
Comparing the release of growth factors when partially demineralized tooth matrix is mixed with platelet rich fibrin and injectable platelet rich fibrin: An in-vitro observational study
- Species:
- Human
- Sample Type:
- Other / Unspecified (PRF, i-PRF, platelet concentrates combined with PDTM)
- Year:
- 2024
- Journal Name:
- Journal of oral and maxillofacial pathology : JOMFP
- PubMed ID:
- 39670126
Hydrogel-Mediated Local Delivery of Induced Nephron Progenitor Cell-Sourced Molecules as a Cell-Free Approach for Acute Kidney Injury
- Species:
- Human
- Sample Type:
- Other / Unspecified (medium conditioned by iNPCs)
- Year:
- 2024
- Journal Name:
- International Journal of Molecular Sciences
- PubMed ID:
- 39408943
Autocrine TGF-alpha is associated with Benzo(a)pyrene-induced mucus production and MUC5AC expression during allergic asthma
- Species:
- Human
- Sample Type:
- NCI-H292 cell
- Year:
- 2022
- Journal Name:
- Ecotoxicology and Environmental Safety
Proteomic aptamer analysis reveals serum markers that characterize preclinical systemic sclerosis (SSc) patients at risk for progression toward definite SSc
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Arthritis Research & Therapy
Long-term repair of porcine articular cartilage using cryopreservable, clinically compatible human embryonic stem cell-derived chondrocytes
- Species:
- Human
- Sample Type:
- Tissue Lysate / Homogenate (lysates of hBMSCs, hES-derived chondrocytes)
- Year:
- 2021
- Journal Name:
- npj Regenerative Medicine
- PubMed ID:
- 34815400
3D printing of silk fibroin-based hybrid scaffold treated with platelet rich plasma for bone tissue engineering.
- Species:
- Human
- Sample Type:
- Plasma (prp)
- Year:
- 2019
- Journal Name:
- Bioactive materials
Transdermal delivery of bFGF with sonophoresis facilitated by chitosan nanocarriers
- Species:
- Mouse
- Sample Type:
- skin tissue
- Year:
- 2022
- Journal Name:
- Journal of Drug Delivery Science and Technology
