Orb778648-96Tests | Human Coxsackie Virus and Adenovirus Receptor

Human Coxsackie Virus and Adenovirus Receptor (CXADR) ELISA Kit

SKU: orb778648

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Description

The Human Coxsackie Virus and Adenovirus Receptor (CXADR) ELISA Kit uses a sandwich enzyme immunoassay to quantify Coxsackie Virus and Adenovirus Receptor (CXADR) in human tissue homogenates, cell lysates and other biological fluids. The kit has a detection range of 0.16-10 ng/mL and a sensitivity of 0.055 ng/mL.

Research Area

Infectious Disease & Virology

Images & Validation

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Tested Applications	ELISA
Application Notes	standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CXADR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CXADR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CXADR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CXADR in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CXADR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CXADR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CXADR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CXADR in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Tissue homogenates, cell lysates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.055 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CAR ELISA Kit, HCAR ELISA Kit, HCVADR ELISA Kit, CVB3-binding protein ELISA Kit, Coxsackievirus B-adenovirus receptor ELISA Kit

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Human CXADR ELISA Kit [orb563003]
Reactivity:
Human
Dynamic Range:
78-5000pg/ml
Sensitivity:
46.9pg/ml
Sizes Available: 96 T, 48 T
Human CXADR ELISA Kit (Ready to Use) [orb550378]
Reactivity:
Human
Dynamic Range:
0.156-10 ng/mL
Sensitivity:
0.065 ng/mL
Sizes Available: 48 T, 96 T
Human CXADR ELISA Kit [orb438975]
Reactivity:
Human
Dynamic Range:
0.156-10 ng/mL
Sensitivity:
0.065 ng/mL
Sizes Available: 48 T, 96 T
Human Coxsackie Virus And Adenovirus Receptor (CXADR) ELISA Kit [orb1666897]
Reactivity:
Human
Sizes Available: 48 T, 96 T