Human insulin-like growth factors 2,IGF-2 ELISA Kit
Référence CSB-E04583h-96T
Conditionnement : 96T
Marque : Cusabio
SDS| Size | 96T,5×96T,10×96T |
Product Details
Target Name
insulin-like growth factor 2 (somatomedin A)
Associated names
C11orf43 ELISA Kit; IGF 2 ELISA Kit; IGF II ELISA Kit; IGF-II ELISA Kit; IGF2 ELISA Kit; IGF2_HUMAN ELISA Kit; IGFII ELISA Kit; INSIGF ELISA Kit; insulin like growth factor 2 (somatomedin A) ELISA Kit; Insulin like Growth Factor 2 ELISA Kit; Insulin like growth factor II ELISA Kit; Insulin like growth factor II precursor ELISA Kit; Insulin like growth factor type 2 ELISA Kit; pp9974 ELISA Kit; Preptin ELISA Kit; putative insulin like growth factor II associated protein ELISA Kit; Somatomedin A ELISA Kit; Somatomedin-A ELISA Kit
Abbreviation
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, cell culture supernates, urine, tissue homogenates
Detection Range
62.5 pg/mL-4000 pg/mL
Sensitivity
15.6 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of human IGF-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 84 | ||||
| Range % | 80-92 | |||||
| 1:400 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:800 | Average % | 97 | ||||
| Range % | 92-104 | |||||
| 1:1600 | Average % | 93 | ||||
| Range % | 86-98 | |||||
Recovery
| The recovery of human IGF-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-104 | ||||
| EDTA plasma (n=4) | 97 | 90-106 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
CUSABIO's IGF2 human ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IGF2 in multiple samples, including serum, plasma, cell culture supernates, urine, and tissue homogenates. This assay uses an antibody specific to human IGF2 coated on a 96-well plate. IGF2 present in a sample is bound to the wells by the immobilized antibody after adding the sample to the wells. Biotinylated IGF2 antibody is added to the wells, forming an antibody-analyte-antibody complex. HRP-conjugated avidin is pipetted to the wells and binds to the complex. A TMB su...
Citations
- M Ding, S Mao, H Wu, S Fang, N Zhen,Advanced Science,2025
- Y Zhang,International immunopharmacology,2023
- Saskia Put.et al,Universiteit Hasselt ,2017
- Dai N.et al,Elife,2017
- Prenatal ambient air pollution and cord blood insulin-like growth factor (IGF) in the ENVIRONAGE birth cohort Saskia .et al,Universiteit Hasselt ,2017
- Yao N et al,Tumour Biol,2012
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