Human Lipoxin A4,LXA4 ELISA Kit
Référence CSB-E09689h-96T
Conditionnement : 96T
Marque : Cusabio
SDS| Size | 96T,5×96T,10×96T |
Product Details
Target Name
Lipoxin A4,LXA4
Abbreviation
LXA4
Species
Homo sapiens (Human)
Sample Types
serum, plasma, cell culture supernates, urine, tissue homogenates, cell lysates
Detection Range
0.625 pg/mL-40 pg/mL
Sensitivity
0.156 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of human LXA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 88 | ||||
| Range % | 84-92 | |||||
| 1:200 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:400 | Average % | 99 | ||||
| Range % | 95-104 | |||||
| 1:800 | Average % | 95 | ||||
| Range % | 88-99 | |||||
Recovery
| The recovery of human LXA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 87-99 | ||||
| EDTA plasma (n=4) | 100 | 95-105 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Human LXA4 ELISA Kit was designed for the quantitative measurement of Human LXA4 protein in serum, plasma, cell culture supernates, urine, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.625 pg/mL-40 pg/mL and the sensitivity is 0.156 pg/mL .
Citations
- J Wang, C Fang, L Quan, Y Zhou,Clinical and Experimental Obstetrics & Gynecology,2025
- Chen YC,Journal of translational medicine,2023
- RM Naguib,The Egyptian Society of Dermatology and Venereology (ESDV),2022
- Chen YC,PLoS One,2019
- Esra Sinem Kemer Dogan,Journal of Periodontal & Implant Science,2019
- Yu D. et al,PLoS One,2015
- Dogan B. et al,Med Sci Monit,2015
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