Specifications

Product Data
Clone Name	HP6025
Application	ELISA, FC, IF, IHC, IP, WB
Recommended Dilution	ELISA: 1/1000-1/2000 (2-9). Immunohistochemistry on Frozen Sections: reported in literature (5,7,10,11). Immunohistochemistry on Paraffin Sections: reported in literature (10). Flow Cytometry: reported in literature (15, 18, 19). Immunocytochemistry: reported in literature (12). Western Blot: reported in literature (3,13-16).
Reactivity	Human
Antibody Host	Mouse
Isotype	IgG1
Clonality	Monoclonal
Immunogen	Human IgG4 myeloma protein
Specificity	This Monoclonal HP6025 antibody recognizes the heavy chain of Human IgG4, at an epitope in the Fc region. No cross-reactivity is observed with IgG1, IgG2, IgG3, IgM, IgA. Cross reactivity with immunoglobulins from other species has not been tested.
Buffer	50mM Tris/ 1 mM MgCl2, 50 % Glycerol, pH 8.0 Label: AP State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide Label: Alkaline Phosphatase
Presentation State	Purified
Concentration	lot specific
Conjugation	AP
Storage	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Shipping	Blue Ice

Product Manuals

Goat anti-Mouse IgG [H&L] Western Blotting Luminol Kit

FAQs

Antibody and Western Standard

SDS

SDS for SM2069AP

Antibody Resources

Product Overview Video: TrueMAB Video Tutorial: Video Tutorial: Antibody specificity and Knockout(KO) lysates Western Blot Protocol IHC Protocol IF Protocol FACS Protocol Immunoprecipitation (IP) Protocol IP protocol with magnetic beads