IgM ELISA Kit (Guinea Pig)
Référence OKIA00037
Conditionnement : 1x96wellplate
Marque : Aviva Systems Biology
IgM ELISA Kit (Guinea Pig) (OKIA00037)
| Datasheets/Manuals | Printable datasheet for IgM ELISA Kit (Guinea Pig) (OKIA00037) |
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| Predicted Species Reactivity | Guinea Pig | ||||||||||||||||
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| Product Format | In Solution | ||||||||||||||||
| Application | ELISA | ||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||
| ELISA Kit Duration | 50 Minutes | ||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology IgM ELISA Kit (Guinea Pig) (OKIA00037) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for IgM has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-IgM - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IgM captured in the well. | ||||||||||||||||
| ELISA Kit Range | 12.5 ng/ml - 400 ng/ml | ||||||||||||||||
| ELISA Kit Component |
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| Additional Information | Intended Use: The IgM test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgM in biological samples of Guinea Pigs. | ||||||||||||||||
| :: | Reagents: 1. DILUENT CONCENTRATE (Running Buffer) One bottle containing 50 ml of a 5X concentrated diluent running buffer. 2. WASH SOLUTION CONCENTRATE One bottle containing 50 ml of a 20X concentrated wash solution. 3. ENZYME-ANTIBODY CONJUGATE 100X One vial containing 150 ul of affinity purified anti-Guinea Pig IgM antibody conjugated with horseradish peroxidase in a stabilizing buffer. 4. CHROMOGEN-SUBSTRATE SOLUTION One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3. 5. STOP SOLUTION One vial containing 12 ml 0.3 M sulfuric acid. WARNING: Avoid contact with skin. 6. ANTI-GUINEA PIG IgM ELISA MICRO PLATE Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Guinea Pig IgM. 7. GUINEA PIG IgM STANDARDS One vial containing Guinea Pig IgM calibrator. | ||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||
| Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||
| Sensitivity | 2.548 ng/ml | ||||||||||||||||
| Predicted Homology Based on Immunogen Sequence | Guinea Pig | ||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Alias Symbols | Ig mu chain C region, IgM, IGHM |
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| Protein Name | IgM |