IL 18 Protein
Référence OPPA00534
Conditionnement : 5µg
Marque : Aviva Systems Biology
| Datasheets/Manuals | Printable datasheet for OPPA00534 |
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| Predicted Species Reactivity | Human |
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| Product Format | Lyophilized 0.5x PBS (pH 7.3) |
| Host | E. Coli |
| Reconstitution and Storage | It is recommended to reconstitute the lyophilized Interleukin 18 in sterile 18M-omega-cm H2O not less than 100 ug/mL, which can then be further diluted to other aqueous solutions. Lyophilized Interleukin 18 although stable at room temperature for 3 weeks, should be stored dessicated below -18°C. Upon reconstitution IL18 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage, it is recommended to add a carrier protein (0.1% HSA and BSA). Please prevent freeze/thaw cycles. |
| Purification | Purified by proprietary chromatographic techniques. |
| Concentration | 1 mg/ml (prior to lyophilization) |
| Purity | Greater than 98.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
| Biological Activity | The ED50 as determined by the dose-dependant stimulation of the IFN-g by human PBMC co-stimulated with IL-12 was found to be 5 ng/ml corresponding to a Specific Activity of 200,000 IU/mg. |
| Gene Symbol | IL18 |
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| Alias Symbols | IGIF, IL-18, IL-1g, IL1F4 |
| NCBI Gene Id | 3606 |
| Protein Name | Interleukin-18 |
| Description of Target | IL-18 is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. Interleukin-18 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 157 amino acids. |
| Uniprot ID | Q14116 |
| Protein Accession # | NP_001553.1 |
| Protein Size (# AA) | Recombinant |
| Molecular Weight | 18 kDa |