IL3 siRNA (Human)
Référence MBS828013-15nmol
Conditionnement : 15nmol
Marque : MyBiosource
IL3 siRNA – Interleukin-3 (Human)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS828013MBS828013 is an siRNA targeting IL3. Also known as IL3 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit IL3 expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
IL3, siRNA
Full Product Name
IL3 siRNA (Human)
Product Gene Name
IL3 sirna
Product Synonym Names
Interleukin-3; IL-3; Hematopoietic growth factor; Mast cell growth factor; MCGF; Multipotential colony-stimulating factor; P-cell-stimulating factor
Host
Synthetic
Species Reactivity
Human
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
IL3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
17,233 Da
OMIM
147740
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human IL3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for IL3 sirna
RNA Interference (RNAi)
Other Notes
Small volumes of IL3 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for IL3. It may not necessarily be applicable to this product.
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
interleukin-3
NCBI Official Synonym Full Names
interleukin 3
NCBI Official Symbol
IL3
NCBI Official Synonym Symbols
IL-3; MCGF; MULTI-CSF
UniProt Protein Name
Interleukin-3
UniProt Synonym Protein Names
Hematopoietic growth factor; Mast cell growth factor; MCGF; Multipotential colony-stimulating factor; P-cell-stimulating factor
Protein Family
Interleukin
UniProt Gene Name
IL3
UniProt Synonym Gene Names
IL-3; MCGF
UniProt Entry Name
IL3_HUMAN
NCBI Protein Information
interleukin-3
NCBI Summary for IL3
The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. [provided by RefSeq, Jul 2008]
Show more UniProt Comments for IL3
IL3: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. Belongs to the IL-3 family.
Protein type: Cell cycle regulation; Oncoprotein; Cytokine; Secreted, signal peptide; Secreted; Apoptosis
Chromosomal Location of Human Ortholog: 5q31.1
Cellular Component: extracellular space; extracellular region
Molecular Function: interleukin-3 receptor binding; growth factor activity; cytokine activity
Biological Process: nervous system development; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of tyrosine phosphorylation of Stat5 protein; cell-cell signaling; embryonic hemopoiesis; cytokine and chemokine mediated signaling pathway; positive regulation of cell proliferation; immune response; positive regulation of myeloid leukocyte differentiation; positive regulation of DNA replication
Show more Protein type: Cell cycle regulation; Oncoprotein; Cytokine; Secreted, signal peptide; Secreted; Apoptosis
Chromosomal Location of Human Ortholog: 5q31.1
Cellular Component: extracellular space; extracellular region
Molecular Function: interleukin-3 receptor binding; growth factor activity; cytokine activity
Biological Process: nervous system development; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of tyrosine phosphorylation of Stat5 protein; cell-cell signaling; embryonic hemopoiesis; cytokine and chemokine mediated signaling pathway; positive regulation of cell proliferation; immune response; positive regulation of myeloid leukocyte differentiation; positive regulation of DNA replication
Research Articles on IL3
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with IL3 sirna
| Reference Product | PubMed Publications |
|---|---|
| IL5 sirna | >50 publications with IL3 and IL5 |
| IL13 sirna | >19 publications with IL3 and IL13 |
| JAK2 sirna | >13 publications with IL3 and JAK2 |
| CSF2RB sirna | >10 publications with IL3 and CSF2RB |
| IL3RA sirna | >8 publications with IL3 and IL3RA |
| FOS sirna | >7 publications with IL3 and FOS |
| STAT5A sirna | >4 publications with IL3 and STAT5A |
Pathways associated with IL3 sirna
| Products by Pathway | Pathway Diagram |
|---|---|
| ARMS-mediated Activation Pathway antibodies | ARMS-mediated Activation Pathway Diagram |
| Apoptosis Pathway antibodies | Apoptosis Pathway Diagram |
| Apoptosis Pathway antibodies | Apoptosis Pathway Diagram |
| Asthma Pathway antibodies | Asthma Pathway Diagram |
| Asthma Pathway antibodies | Asthma Pathway Diagram |
| Axon Guidance Pathway antibodies | Axon Guidance Pathway Diagram |
| Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies | Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram |
| Calcium Signaling In The CD4+ TCR Pathway antibodies | Calcium Signaling In The CD4+ TCR Pathway Diagram |
| Cytokine Signaling In Immune System Pathway antibodies | Cytokine Signaling In Immune System Pathway Diagram |
| Cytokine-cytokine Receptor Interaction Pathway antibodies | Cytokine-cytokine Receptor Interaction Pathway Diagram |
Diseases associated with IL3 sirna
| Disease Name | Pubmed Publications |
|---|---|
| Necrosis Antibodies | >32 publications with IL3 and Necrosis |
| Disease Models, Animal Antibodies | >31 publications with IL3 and Disease Models, Animal |
| Lung Diseases Antibodies | >24 publications with IL3 and Lung Diseases |
| Inflammation Antibodies | >23 publications with IL3 and Inflammation |
| Neoplasms, Experimental Antibodies | >21 publications with IL3 and Neoplasms, Experimental |
| Asthma Antibodies | >17 publications with IL3 and Asthma |
| Anemia Antibodies | >12 publications with IL3 and Anemia |
| Nervous System Diseases Antibodies | >11 publications with IL3 and Nervous System Diseases |
| Brain Diseases Antibodies | >8 publications with IL3 and Brain Diseases |
| Skin Neoplasms Antibodies | >6 publications with IL3 and Skin Neoplasms |
Organs/Tissues associated with IL3 sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Mixed Antibodies | >13 publications with IL3 and Mixed |