Inositol trisphosphate ELISA Kit
Référence OKEH02606
Conditionnement : 96Wells
Marque : Aviva Systems Biology
Inositol trisphosphate ELISA Kit (OKEH02606)
| Datasheets/Manuals | Printable datasheet for Inositol trisphosphate ELISA Kit (OKEH02606) |
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| Predicted Species Reactivity | All Species | ||||||||||||||||||||||
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| Application | ELISA-Comp | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology Inositol trisphosphate ELISA Kit (OKEH02606) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Inositol trisphosphate antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Inositol trisphosphate and incubated. The Inositol trisphosphate found in the sample or standards competes with the biotinylated Inositol trisphosphate for limited binding sites on the immobilized anti-Inositol trisphosphate antibody. Excess unbound biotinylated Inositol trisphosphate and sample or standard Inositol trisphosphate is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Inositol trisphosphate captured in the well and inversely proportional to the amount of Inositol trisphosphate which was contained in the sample or standard. | ||||||||||||||||||||||
| ELISA Kit Range | 0.625-40ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into Serum and Plasma = 90% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: < 4.9% (n = 20) Mean Inter-assay CV%: < 8.3% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Component |
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| Additional Information | Target Abbr: IP3 Target Name: Inositol trisphosphate | ||||||||||||||||||||||
| :: | Pubchem: 439456 | ||||||||||||||||||||||
| :: | Chemical formula: C6H15O15P3 | ||||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 0.265 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant General Inositol trisphosphate | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Competitive ELISA |
| Alias Symbols | IP3, Inositol trisphosphate, IP3, Inositol 1, 4, 5-trisphosphate |
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| Protein Name | Inositol trisphosphate |
| Description of Target | Inositol trisphosphate or inositol 1,4,5-trisphosphate (also commonly known as triphosphoinositol; abbreviated InsP3 or IP3), together with diacylglycerol (DAG), is a secondary messenger molecule used in signal transduction and lipid signaling in biological cells. While DAG stays inside the membrane, IP3 is soluble and diffuses through the cell. It is made by hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2), a phospholipid that is located in the plasma membrane, by phospholipase C (PLC). |