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> Integrin alpha V beta 8 Protein, Human, Recombinant (His)

Integrin alpha V beta 8 Protein, Human, Recombinant (His)

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Référence NB-64-60605-10ug

Conditionnement : 10ug

Marque : Neo Biotech


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Integrin alpha V beta 8 Protein, Human, Recombinant (His)

(Synonyms: Integrin α V β 8, Integrin alpha V β8) Copy Product Info

Synonyms: Integrin α V β 8, Integrin alpha V β8

Catalog No. TMPY-05717 Copy Product Info
Integrin alpha V beta 8 is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligands. ITGAVB8 does not appear to assume different activation states; and the cytoplasmic tail does not connect to the cytoskeleton. It binds ligands containing an RGD motif; including vitronectin; fibrin and the latency associated peptide (LAP) of the latent TGF-beta complex. High affinity binding of alpha V beta 8 to LAP allows proteolytic cleavage by MT1-MMP; which releases active TGF-beta. This mechanism differs from that of alpha V beta 6; the other alpha V integrin which can activate TGF-beta from latency through non-proteolytic mechanisms.
For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:SDS-PAGE: 97.1%; SEC-HPLC: 96.8%
Appearance:Lyophilized powder
Color:White
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Product Information

Bioactivity
Bioactivity
Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
Integrin alpha V beta 8 is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligands. ITGAVB8 does not appear to assume different activation states; and the cytoplasmic tail does not connect to the cytoskeleton. It binds ligands containing an RGD motif; including vitronectin; fibrin and the latency associated peptide (LAP) of the latent TGF-beta complex. High affinity binding of alpha V beta 8 to LAP allows proteolytic cleavage by MT1-MMP; which releases active TGF-beta. This mechanism differs from that of alpha V beta 6; the other alpha V integrin which can activate TGF-beta from latency through non-proteolytic mechanisms.
Species
Human
Expression System
HEK293 Cells
TagC-His
Accession NumberP06756&P26012
ConstructionA DNA sequence encoding the extracellular domain (Met1-Val992) of human ITGAV (P06756) was fused with a C-terminal polyhistidine tag was expressed., constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Arg684) of human ITGB8 (P26012) constructed the plasmid 2. The two plasmids were co-expressed and the human ITGAV&ITGB8 heterodimer was purified. Predicted N terminal: Phe31 & Glu43
Protein Purity
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
Integrin alpha V beta 8 Protein, Human, Recombinant (His),Integrin alpha V beta 8 Protein, Human, Recombinant (His)
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionReconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
SynonymsIntegrin α V β 8, Integrin alpha V β8
Research Background
Integrin alpha V beta 8 is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligands. ITGAVB8 does not appear to assume different activation states; and the cytoplasmic tail does not connect to the cytoskeleton. It binds ligands containing an RGD motif; including vitronectin; fibrin and the latency associated peptide (LAP) of the latent TGF-beta complex. High affinity binding of alpha V beta 8 to LAP allows proteolytic cleavage by MT1-MMP; which releases active TGF-beta. This mechanism differs from that of alpha V beta 6; the other alpha V integrin which can activate TGF-beta from latency through non-proteolytic mechanisms.
Chemical Properties
Molecular Weight187.95 kDa (predicted)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Related Tags: Integrin alpha V beta 8 Protein, Human, Recombinant (His) chemical structure | Integrin alpha V beta 8 Protein, Human, Recombinant (His) molecular weight