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> Interleukin 5 (IL-5, BCDF-alpha, Eosinophil Differentiation Factor, EDF) (Biotin)

Interleukin 5 (IL-5, BCDF-alpha, Eosinophil Differentiation Factor, EDF) (Biotin)

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Référence I8427-02A-250ug

Conditionnement : 250ug

Marque : US Biological


I8427-02A Interleukin 5 (IL-5, BCDF-alpha, Eosinophil Differentiation Factor, EDF) (Biotin)

Clone Type
Monoclonal
Host
mouse
Source
feline
Swiss Prot
O77515
Isotype
IgG1
Grade
Affinity Purified
Applications
E
Crossreactivity
Fe
Shipping Temp
Blue Ice
Storage Temp
-20°C

Interleukin-5 (IL-5) is a 40kD secreted, heparin-binding, disulfide-linked homodimeric glycoprotein that belongs to the a-helical group of cytokines. It promotes the proliferation, differentiation and activation of eosinophils. The 115aa mature feline IL-5 shares 70-59aa sequence identity with human and mouse IL-5, respectively. The IL-5 receptor is a complex of an IL-5-specific ligand-binding a subunit with a signal transducing common b subunit (bc) that is shared with receptor complexes for IL-3 and GM-CSF.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA (capture): 2-8ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 11n97|Host: mouse|Source: feline|Concentration: ~0.5mg/ml (after reconstitution) |Form: Supplied as a lyophilized powder in PBS, pH 7.4, BSA. Reconstitute with sterile 500ul PBS. Labeled with Biotin.|Purity: Purified by Protein G affinity chromatography from hybridoma culture supernatant.|Immunogen: Recombinant protein corresponding to Ile20-Ser134 from feline IL-5, expressed in NSO cells.|Specificity: Recognizes feline IL-5. Does not react with feline GM-CSF or recombinant equine, canine, mouse, human or porcine IL-5.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Recombinant protein corresponding to Ile20-Ser134 from feline IL-5, expressed in NSO cells.
Form
Supplied as a lyophilized powder in PBS, pH 7.4, BSA. Reconstitute with sterile 500ul PBS. Labeled with Biotin.
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Specificity
Recognizes feline IL-5. Does not react with feline GM-CSF or recombinant equine, canine, mouse, human or porcine IL-5.