IPP siRNA (Human)
Référence MBS827226-30nmol
Conditionnement : 30nmol
Marque : MyBiosource
IPP siRNA – Actin-Binding Protein IPP Isoform 2 (Human)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS827226MBS827226 is an siRNA targeting IPP. Also known as IPP siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit IPP expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
IPP, siRNA
Full Product Name
IPP siRNA (Human)
Product Gene Name
IPP sirna
Product Synonym Names
KLHL27; Actin-binding protein IPP; Intracisternal A particle-promoted polypeptide; IPP; Kelch-like protein 27
Host
Synthetic
Species Reactivity
Human
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
IPP siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
65,592 Da
OMIM
147485
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human IPP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for IPP sirna
RNA Interference (RNAi)
Other Notes
Small volumes of IPP sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for IPP. It may not necessarily be applicable to this product.
NCBI Official Full Name
actin-binding protein IPP isoform 2
NCBI Official Synonym Full Names
intracisternal A particle-promoted polypeptide
NCBI Official Symbol
IPP
NCBI Official Synonym Symbols
KLHL27
UniProt Protein Name
Actin-binding protein IPP
UniProt Synonym Protein Names
Intracisternal A particle-promoted polypeptide; IPP; Kelch-like protein 27
Protein Family
Actin-binding protein
UniProt Gene Name
IPP
UniProt Synonym Gene Names
KLHL27; IPP
UniProt Entry Name
IPP_HUMAN
NCBI Protein Information
actin-binding protein IPP
NCBI Summary for IPP
The protein encoded by this gene is a member of the kelch family of proteins, which is characterized by a 50 amino acid repeat which interacts with actin. Transcript variants have been described but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]
UniProt Comments for IPP
IPP: May play a role in organizing the actin cytoskeleton. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 1p34-p32
Cellular Component: cytoplasm; actin cytoskeleton
Molecular Function: actin binding
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 1p34-p32
Cellular Component: cytoplasm; actin cytoskeleton
Molecular Function: actin binding
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with IPP sirna
| Reference Product | PubMed Publications |
|---|---|
| CD69 sirna | >11 publications with IPP and CD69 |
| IDI1 sirna | >5 publications with IPP and IDI1 |
| ISPD sirna | >4 publications with IPP and ISPD |
| PARVA sirna | >4 publications with IPP and PARVA |
| CCR5 sirna | >3 publications with IPP and CCR5 |
| FDPS sirna | >2 publications with IPP and FDPS |
| FGF9 sirna | >1 publications with IPP and FGF9 |
Diseases associated with IPP sirna
| Disease Name | Pubmed Publications |
|---|---|
| Nervous System Diseases Antibodies | >83 publications with IPP and Nervous System Diseases |
| Kidney Diseases Antibodies | >51 publications with IPP and Kidney Diseases |
| Hypertension Antibodies | >42 publications with IPP and Hypertension |
| Inflammation Antibodies | >32 publications with IPP and Inflammation |
| Disease Models, Animal Antibodies | >30 publications with IPP and Disease Models, Animal |
| Necrosis Antibodies | >26 publications with IPP and Necrosis |
| Brain Diseases Antibodies | >24 publications with IPP and Brain Diseases |
| Fibrosis Antibodies | >20 publications with IPP and Fibrosis |
| Liver Diseases Antibodies | >15 publications with IPP and Liver Diseases |
| Neoplasms, Experimental Antibodies | >14 publications with IPP and Neoplasms, Experimental |
Organs/Tissues associated with IPP sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Prostate Antibodies | >64 publications with IPP and Prostate |
| Bone Antibodies | >64 publications with IPP and Bone |
| Kidney Antibodies | >57 publications with IPP and Kidney |
| Skin Antibodies | >47 publications with IPP and Skin |
| Muscle Antibodies | >47 publications with IPP and Muscle |
| Lung Antibodies | >44 publications with IPP and Lung |
| Brain Antibodies | >43 publications with IPP and Brain |
| Liver Antibodies | >37 publications with IPP and Liver |
| Heart Antibodies | >34 publications with IPP and Heart |
| Pancreas Antibodies | >28 publications with IPP and Pancreas |