LILRB1/CD85j/ILT2 Protein, Human, Recombinant (His & Avi)
Référence NB-64-54808-50ug
Conditionnement : 50ug
Marque : Neo Biotech
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LILRB1/CD85j/ILT2 Protein, Human, Recombinant (His & Avi)
(Synonyms: XXbac-BCX85G21.4, MIR7, LIR1, LILRB1, ILT2FLJ37515, ILT-2, ILT2, CD85J) Copy Product InfoSynonyms: XXbac-BCX85G21.4, MIR7, LIR1, LILRB1, ILT2FLJ37515, ILT-2, ILT2, CD85J
Catalog No. TMPK-01107 Copy Product Info
LILRB1/CD85j/ILT2 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 50 kDa and the accession number is Q8NHL6-1.
For research use only—not for human use. No sales to individuals. Use as intended only.
Product Information
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Immobilized Human LILRB1, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-LILRB1 Antibody, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA. |
| Description | LILRB1/CD85j/ILT2 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 50 kDa and the accession number is Q8NHL6-1. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-His-Avi |
| Accession Number | Q8NHL6-1 |
| Construction | Gly24-His458 |
| Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Synonyms | XXbac-BCX85G21.4, MIR7, LIR1, LILRB1, ILT2FLJ37515, ILT-2, ILT2, CD85J |
| Research Background | LILRB1, also known as CD85j and ILT2, is a 110 kDa transmembrane glycoprotein in the LILR immunoregulatory protein family. Mature human LILRB1 consists of a 438 amino acid (aa) extracellular domain (ECD) with 4 tandem Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 4 inhibitory ITIM motifs.LILRB1 is a receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles. Receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response. |
| Molecular Weight | 50 kDa (predicted). Due to glycosylation, the protein migrates to 72-78 kDa based on Tris-Bis PAGE result. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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Keywords
MIR-7MIR 7LIR-1LIR 1LILRB-1LILRB 1ILT2FLJ-37515ILT2FLJ 37515ILT 2
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Related Tags: LILRB1/CD85j/ILT2 Protein, Human, Recombinant (His & Avi) chemical structure | LILRB1/CD85j/ILT2 Protein, Human, Recombinant (His & Avi) molecular weight
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