Orb1409947 | Magic Red Cathepsin B Assay Kit Clinisciences

Magic Red Cathepsin B Assay Kit

SKU: orb1409947

Description

Quantitatively monitor intracellular cathepsin B activity over time in vitro. The Magic Red substrate fluoresces red upon cleavage by active cathepsin enzymes. The signal can be analyzed using fluorescence microscopy or a fluorescence plate reader.

Images & Validation

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Application Notes	1. Prepare samples and controls. 2. Reconstitute Magic Red by adding 50 µL DMSO. 3. Dilute Magic Red 1:10 by adding 450 µL diH2O. 4. Add 20 µL Magic Red to each sample (~ 500 µL aliquot of cultured cells). 5. Incubate while protected from light. 6. Watch color start to develop within 15 minutes. 7. If desired, label with additional stains, such as Hoechst, DAPI, Acridine orange, or an antibody. 8. If desired, fix or embed cells. 9. Analyze with a fluorescence microscope or fluorescence plate reader. Magic Red has an optimal excitation at 592 nm and emission at 628 nm. If working with adherent cells, please see the manual for additional protocols.

Application Notes

1. Prepare samples and controls. 2. Reconstitute Magic Red by adding 50 µL DMSO. 3. Dilute Magic Red 1:10 by adding 450 µL diH2O. 4. Add 20 µL Magic Red to each sample (~ 500 µL aliquot of cultured cells). 5. Incubate while protected from light. 6. Watch color start to develop within 15 minutes. 7. If desired, label with additional stains, such as Hoechst, DAPI, Acridine orange, or an antibody. 8. If desired, fix or embed cells. 9. Analyze with a fluorescence microscope or fluorescence plate reader. Magic Red has an optimal excitation at 592 nm and emission at 628 nm. If working with adherent cells, please see the manual for additional protocols.

Key Properties

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Sample Types	Cell culture

Storage & Handling

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Storage	2-8°C
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

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Magic Red Cathepsin B Assay Kit (orb1409947)

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Magic Red Cathepsin B Assay Kit

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Magic Red Cathepsin B Assay Kit

Description

Images & Validation

Key Properties

Storage & Handling

Documents Download

Request a Document

Protocol Information

Magic Red Cathepsin B Assay Kit (orb1409947)