Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.
 
> Mix-n-Stain™ CF®405S Antibody Labeling Kit, 1x(50-100ug) labeling

Mix-n-Stain™ CF®405S Antibody Labeling Kit, 1x(50-100ug) labeling

Imprimer Imprimer
Identifiez-vous

Référence 92231

Conditionnement : 1kit

Marque : Biotium

Mix-n-Stain™ CF®405S Antibody Labeling Kit, 1x(50-100ug) labeling

Mix-n-Stain™ CF® Dye Antibody Labeling Kits

Label your IgG antibody with one of our superior CF® Dyes in as little as 15 minutes without a purification step. Labeling tolerates many common buffer components including BSA and ascites.

Add to Cart
This product is available by special order only.

Please fill in the inquiry form and we will contact you shortly.
Don't see what you're looking for? Contact us for more conjugation options.
Request Quote or Bulk Order
Mix-n-Stain™ CF® Dye Antibody Labeling Kits
Overview of Mix-n-Stain Labeling ProtocolOverview of the Mix-n-Stain™ Antibody Labeling Kit workflow.
Mix-n-Stain™ CF® Dye Antibody Labeling Kits - Image 3SEC-purified MCF-7-derived exosomes were stained with CD9 monoclonal antibody (CD9/2343) labeled with CF®488A using a Mix-n-Stain™ kit (right). Specific staining was seen, compared with the same antibody in buffer (left). Exosomes were detected on a CytoFLEX LX flow cytometer in the FITC channel.
Mix-n-Stain™ CF® Dye Antibody Labeling Kits - Image 4Flow cytometry of MCF-7 cells unstained (gray) or stained with CD9 monoclonal antibody (CD9/2343) labeled with CF®488A using a Mix-n-Stain™ kit (green).
Mix-n-Stain™ CF® Dye Antibody Labeling Kits - Image 5Mouse anti-transferrin receptor antibody from (endosome and plasma membrane marker) was labeled using Lightning-Link® Rapid DyLight® Conjugation Kits from Novus Biologicals (left) or Mix-n-Stain™ CF® Dye Antibody Labeling Kits (right) according to manufacturers’ instructions. The CF® dye conjugates show higher signal and more specific staining compared the DyLight®conjugates when imaged using the same settings, due to the superior brightness of CF® dyes. The insets show the same field of view imaged with a higher gain setting.
Mix-n-Stain™ CF® Dye Antibody Labeling Kits - Image 6Mix-n-Stain™ labeling can be performed on unpurified antibodies in ascites fluid. Top: HeLa cells stained with anti-tubulin antibody in ascited, detected with CF®488A secondary antibody (left) or directly labeled using CF®488A Mix-n-Stain™ (right). Bottom: rat optic nerve cryosections stained with anti-GFAP (glial marker) detected with CF®568 secondary antibody or directly labeled with Mix-n-Stain™ CF®568. antibodies in Mix-n-Stain™ labeled ascites show comparable specificity to unlabeled antibodies detected using secondary antibody.
Mix-n-Stain™ CF® Dye Antibody Labeling Kits - Image 7Monoclonal mouse anti-tubulin clone DM1A in ascites or purified in PBS with 1% BSA was labeled with CF®488A Mix-n-Stain using the modified protocol for total protein labeling. The Mix-n-Stain antibodies performed comparably in immunofluorescence staining of HeLa cells as unlabeled antibody (in ascites) detected with secondary antibody.
combined direct and indirect immunofluorescence staining of rat testis for microtubules, actin filaments and tight junctionsRat testis with mouse anti-tubulin and CF®488A goat anti-mouse (min x rat) (green), CF®555 Mix-n-Stain labeled mouse anti-ZO1 (tight junctions, red) and CF®640R phalloidin (cyan).
Mix-n-Stain™ CF® Dye Antibody Labeling Kits - Image 9HeLa cells stained with CF640R Mix-n-Stain labeled mouse anti-tubulin antibody (red). Actin filaments are stained with CF488A phalloidin (green). Mounted in Everbrite Mounting Medium with DAPI (nuclei, blue).
Mix-n-Stain™ CF® Dye Antibody Labeling Kits - Image 10Mix-n-Stain™ labeling in the presence of 10-fold excess BSA or gelatin to IgG by weight using the modified Mix-n-Stain™ protocol. Mouse anti-transferrin receptor IgG or mouse IgG isotype control from BD Pharmingen were labeled using CF647 Mix-n- Stain™ kits. Intracellular staining of Jurkat cells was analyzed in channel FL3 of a BD FACSCalibur flow cytometer. Antibodies labeled in the presence of stabilizer showed comparable signal to noise ratio compared to conjugates labeled without stabilizer. A. Graph shows the geometric mean fluorescence of the cell populations. B. Graph shows signal to noise ratio (anti-TfR fluorescence/isotype fluorescence).
Mix-n-Stain™ CF® Dye Antibody Labeling Kits - Image 11Comparison of Mix-n-Stain™ CF®647 labeling of anti-TfR antibody (endosome and cell surface marker) in HeLa cells without added protein compared to 1% BSA or 1% gelatin (10-fold excess) using the modified protocol for labeling in the presence of stabilizer protein. Labeling using the modified protocol gives comparable results as the standard protocol, with bright signal and minimal increase in non-specific background.
Mix-n-Stain™ CF® Dye Antibody Labeling Kits - Image 12Mix-n-Stain™ Compatibility and Protocol Selection flowchart. Click to enlarge.
View Spectra Viewer
Antibody Finder
Request Custom Conjugation

Product Description

Mix-n-Stain™ CF® Dye Antibody Labeling Kits allow you to label ≤5 ug up to 100 ug of your IgG antibody in as little as 15 minutes, with minimal hands-on time and no purification.

Note: This kit is for labeling IgG antibodies only. For labeling IgM antibodies, please see our Mix-n-Stain™ CF® Dye IgM Antibody Labeling Kits.

Features

  • Label 5-20 ug, 20-50 ug, or 50-100 ug Ab
  • Choice of over 30 CF® Dye colors
  • Less than 30 seconds of hands-on time
  • 15 minutes total reaction time
  • No purification, 100% recovery
  • Compatible with BSA, gelatin, ascites

Kit components

  • Ultrafiltration spin vial (for antibody concentration or buffer exchange, if needed)
  • 10X Mix-n-Stain™ Reaction Buffer
  • Lyophilized Reactive CF® Dye
  • Mix-n-Stain™ Storage Buffer
Overview of Mix-n-Stain Labeling Protocol

Overview of the Mix-n-Stain™ Antibody Labeling Kit workflow. Click to enlarge.

Unrivaled Convenience

Mix-n-Stain™ labeling tolerates common antibody buffer formulations. An ultrafiltration centrifuge vial is provided to quickly remove interfering substances like glycerol, or to concentrate your antibody. With a slight modification in protocol, antibodies can be labeled in the presence of BSA, gelatin, or ascites fluid. Because CF® Dyes are highly water soluble, the presence of other proteins like BSA or gelatin in the labeling reaction has minimal effect on background fluorescence, because any labeled non-antibody proteins readily wash away during immunofluorescence staining.

Superior CF® Dyes

These kits feature a choice of 31 of Biotium's next-generation CF® Dyes, which were designed for advantages in brightness and photostability compared to other commercially available fluorescent dyes. Learn more about CF® Dyes.

Choose the Right Labeling Kit for Your Antibody

Mix-n-Stain™ Antibody Labeling Kits are very simple to use (see the workflow overview above). But before you begin, you must check that your antibody meets the compatibility requirements for labeling, and choose the right labeling protocol. Download the updated and expanded Product Protocol for a labeling check list to help you select the right kit size and labeling protocol for your antibody. Also see our Kit Compatibility and Protocol Selection Flowchart.

See the product table below for a quick reference of the maximum excitation and emission of each CF® Dye, or see our CF® Dye Brochure for more detailed dye information.

Other Labeling Kits

We now offer Mix-n-Stain™ Maxi Antibody Labeling Kits for labeling 1 mg of IgG antibody with CF® Dyes, as well as Mix-n-Stain™ CF® Dye IgM Antibody Labeling Kits and Mix-n-Stain™ Nanobody Labeling Kits. Mix-n-Stain™ kits also are available for labeling antibodies with biotin, DNP, or DIG. See our full selection of Mix-n-Stain™ Antibody Labeling Kits.

Mix-n-Stain™ CF® Dye Antibody Labeling Kits

ConjugationEx/EmSizeCatalog No.Dye Features
CF®350347/448 nm5-20 ug92270CF®350 Features
20-50 ug92250
50-100 ug92230
CF®405S404/431 nm5-20 ug92271CF®405S Features
20-50 ug92251
50-100 ug92231
CF®405M408/452 nm5-20 ug92272CF®405M Features
20-50 ug92252
50-100 ug92232
CF®405L395/545 nm5-20 ug92303CF®405L Features
20-50 ug92304
50-100 ug92305
3x(5-20 ug)92445
CF®430426/498 nm5-20 ug92316CF®430 Features
20-50 ug92317
50-100 ug92318
CF®440440/515 nm5-20 ug92319CF®440 Features
20-50 ug92320
50-100 ug92321
CF®450450/538 nm5-20 ug92322CF®450 Features
20-50 ug92323
50-100 ug92324
CF®488A490/515 nm5-20 ug92273CF®488A Features
20-50 ug92253
50-100 ug92233
3x(5-20 ug)92446
CF®514516/548 nm5-20 ug92331CF®514 Features
20-50 ug92332
50-100 ug92333
CF®532527/558 nm5-20 ug92289CF®532 Features
20-50 ug92290
50-100 ug92291
CF®543541/560 nm5-20 ug92287CF®543 Features
20-50 ug92267
50-100 ug92247
CF®555555/565 nm5-20 ug92274CF®555 Features
20-50 ug92254
50-100 ug92234
CF®568562/583 nm5-20 ug92275CF®568 Features
20-50 ug92255
50-100 ug92235
3x(5-20 ug)92447
CF®570568/591 nm5-20 ug92334CF®570 Features
20-50 ug92335
50-100 ug92336
CF®583583/606 nm5-20 ug92337CF®583 Features
20-50 ug92338
50-100 ug92339
CF®583R586/609 nm5-20 ug92455CF®583R Features
20-50 ug92456
50-100 ug92457
CF®594593/614 nm5-20 ug92276CF®594 Features
20-50 ug92256
50-100 ug92236
3x(5-20 ug)92448
CF®597R597/619 nm5-20 ug92461CF®597R Features
20-50 ug92462
50-100 ug92463
CF®633630/650 nm5-20 ug92277CF®633 Features
20-50 ug92257
50-100 ug92237
CF®640R642/662 nm5-20 ug92278CF®640R Features
20-50 ug92258
50-100 ug92245
CF®647650/665 nm5-20 ug92279CF®647 Features
20-50 ug92259
50-100 ug92238
3x(5-20 ug)92449
CF®660R663/682 nm5-20 ug92281CF®660R Features
20-50 ug92261
50-100 ug92243
CF®660C667/685 nm5-20 ug92280CF®660C Features
20-50 ug92260
50-100 ug92239
CF®680681/698 nm5-20 ug92282CF®680 Features
20-50 ug92262
50-100 ug92240
CF®680R680/701 nm5-20 ug92283CF®680R Features
20-50 ug92263
50-100 ug92246
CF®700695/720 nm5-20 ug92425CF®700 Features
20-50 ug92426
50-100 ug92427
CF®710712/736 nm5-20 ug92579CF®710 Features
20-50 ug92580
50-100 ug92581
CF®725729/750 nm5-20 ug92582CF®725 Features
20-50 ug92583
50-100 ug92584
CF®740742/767 nm5-20 ug92576CF®740 Features
20-50 ug92577
50-100 ug92578
CF®750755/779 nm5-20 ug92284CF®750 Features
20-50 ug92264
50-100 ug92241
CF®770770/797 nm5-20 ug92285CF®770 Features
20-50 ug92265
50-100 ug92242
CF®790784/806 nm5-20 ug92288CF®790 Features
20-50 ug92268
50-100 ug92248
CF®800797/816 nm5-20 ug92428CF®800 Features
20-50 ug92429
50-100 ug92430
CF®820822/835 nm5-20 ug92431CF®820 Features
20-50 ug92432
50-100 ug92433
* Download the Product Protocol for a labeling check list to help you select the right kit size and labeling protocol for your antibody.
Also see our Kit Compatibility and Protocol Selection Flowchart.

Antibody & Protein Labeling Kits

Product
Name		Label
Options		Labeling
Scale		Labeling
Time		Features
Mix-n-Stain™ CF® Dye
Antibody Labeling Kits		CF® Dyes≤5-20 ug IgG
20-50 ug IgG
50-100 ug IgG		~ 15 min.• Rapid, simple labeling

• No purification

• Tolerates BSA & other additives

• Dyes for super-resolution, spectral flow, & NIR detection
Mix-n-Stain™ FITC
Antibody Labeling Kits		FITC
Mix-n-Stain™ Cyanine Dye
Antibody Labeling Kits		Cyanine 555
Cyanine 647
Mix-n-Stain™ Biotin
Antibody Labeling Kits		Biotin
Mix-n-Stain™ Digoxigenin
Antibody Labeling Kits		Digoxygenin (DIG)
Mix-n-Stain™ DNP
Antibody Labeling Kits		DNP
Mix-n-Stain™ Maxi
Antibody Labeling Kits		CF® Dyes
Cyanine Dyes		1 mg IgG~ 30 min.
Mix-n-Stain™ CF® Dye IgM Antibody Labeling KitsCF® Dyes
FITC		25 ug IgM
100 ug IgM		15-30 min.• Rapid, simple labeling

• No purification
Mix-n-Stain™ STORM CF® Dye Antibody Labeling KitsCF® Dyes50 ug IgG~ 30 min.• Optimized to provide low 1-2.5 DOL

• No purification
Mix-n-Stain™
Nanobody Labeling Kits		CF® Dyes5-20 ug Nanobody®
20-50 ug Nanobody®		~ 30 min.• Optimized for Nanobodies®

• Tolerates BSA & other additives
Mix-n-Stain™
Nanobody Thiol Labeling Kits		CF® Dyes~ 2 hours• Optimized for Nanobodies® with single exposed cysteine residue
Mix-n-Stain™
Small Ligand Labeling Kits		CF® Dyes0.1 umol
small ligand		~ 30 min.• Label SNAP®, CLIP™, HaloTag® & other small ligands

• Dye options for surface or intracellular targets
VivoBrite™ Rapid Antibody Labeling Kits for Small Animal In Vivo ImagingNear-IR CF® Dyes3 x 1 mg IgG~ 2 hours• Dyes, buffers, & spin vials for labeling + purification

• Sterile syringes, filters and storage vials for in vivo use
CF® Dye & Biotin SE
Protein Labeling Kits		CF® Dyes
Biotin		3 x 1 mg
protein		~ 2 hours• Dyes, buffers, & spin vials for labeling + purification
Alexa Fluor and DyLight are registered trademarks of Thermo Fisher Scientific.
Technology
Technology Icon
CF® Dyes
Technology Icon
CF® Dyes for Multi-Color Super-Resolution Microscopy
Technology Icon
Mix-n-Stain™ CF® Dye Antibody Labeling Kits

Product Attributes

Size
3x(5-20 ug) labelings, 5-20 ug labeling, 20-50 ug labeling, 50-100 ug labeling

Documents, Protocols, SDS and COA

References

Download a list of CF® Dye and Mix-n-Stain™ references.

Citations

Download a list of CF® Dye and Mix-n-Stain™ references.

Bioz Powered by Bioz See more details on Bioz

Vous serez peut-être également intéressé par les produits suivants :



Référence
Description
Cond.
Prix HT
4030-30
Goat Anti-Rabbit IgG-AF488
 1.0mg 
MBS224741-0,2mg
MOUSE ANTI HUMAN CD16
 0.2mg