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ML-178 [1355026-47-9]

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Référence NB-64-11430-1mL

Conditionnement : 1mLx10mM(inDMSO)

Marque : Neo Biotech

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ML-178

(Synonyms: CYM50179) Copy Product Info

Synonyms: CYM50179

Catalog No. T22102 Copy Product Info

Purity: 95.92%

ML-178, a dichlorobenzene, is a potent and selective agonist of sphingosine-1-phosphate receptor 4 (S1PR4), with an EC50 of 46 nM, and has no effect on other S1P receptors at a concentration of 25 μM.

Cas No. 1355026-47-9

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:95.92%

Product Introduction

ML-178 AI Summary

ML-178 is identified as a potent agonist of the Sphingosine 1-Phosphate Receptor 4 (S1P4) with an EC50 value of approximately 46 nM. It demonstrates high selectivity for S1P4, showing no significant bioactivity against S1P1, S1P2, and S1P3 receptors in respective counterscreen assays. The compound was tested in human U2OS cells, which express the S1P4 receptor along with the EDG6 gene, GAL4-VP16, and beta-arrestin/TEV protease, using a FRET-based beta-lactamase reporter gene assay to confirm its activity via nuclear migration of VP16-GAL4. Additionally, ML-178 exhibits low cytotoxicity, with a CC50 value exceeding 20,000 nM, indicating its potential safety at high concentrations..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	ML-178, a dichlorobenzene, is a potent and selective agonist of sphingosine-1-phosphate receptor 4 (S1PR4), with an EC50 of 46 nM, and has no effect on other S1P receptors at a concentration of 25 μM.
Targets & IC50	S1P4 receptor:46 nM (EC50)
Synonyms	CYM50179

Chemical Properties

Molecular Weight	441.93
Formula	C₁₃H₉Br₂Cl₂NO₂
Cas No.	1355026-47-9
Smiles	Clc1ccc(OCCOc2ccc(Br)nc2Br)c(Cl)c1
Relative Density.	no data available

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 15 mg/mL (33.94 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.2628 mL	11.3140 mL	22.6280 mL	113.1401 mL
5 mM	0.4526 mL	2.2628 mL	4.5256 mL	22.6280 mL
10 mM	0.2263 mL	1.1314 mL	2.2628 mL	11.3140 mL
20 mM	0.1131 mL	0.5657 mL	1.1314 mL	5.6570 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

Keywords

viral infectionsML-178ML178ML 178Lysophospholipid ReceptorLPL ReceptorInhibitorinhibithrombocytopeniaCYM-50179CYM 50179autoimmune diseases

Related Tags: ML-178 chemical structure | ML-178 formula | ML-178 molecular weight