MMP-13 Chemi-Luminescent ELISA Kit (Chicken)
Référence OKCD03771
Conditionnement : 96Wells
Marque : Aviva Systems Biology
MMP-13 Chemi-Luminescent ELISA Kit (Chicken) (OKCD03771)
| Datasheets/Manuals | Printable datasheet for MMP-13 Chemi-Luminescent ELISA Kit (Chicken) (OKCD03771) |
|---|
| Predicted Species Reactivity | Chicken | ||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Application | CLIA | ||||||||||||||||||||||
| ELISA Kit Detection Method | Chemiluminescent | ||||||||||||||||||||||
| ELISA Kit Duration | 2h, 40min | ||||||||||||||||||||||
| ELISA Kit Linearity |
| ||||||||||||||||||||||
| ELISA Kit Principle | The microplate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 13 (MMP13). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 13 (MMP13). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Matrix Metalloproteinase 13 (MMP13) level in the sample or standard. | ||||||||||||||||||||||
| ELISA Kit Range | 0.14-100ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery |
| ||||||||||||||||||||||
| ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 13 (MMP13) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 13 (MMP13) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
| ELISA Kit Component |
| ||||||||||||||||||||||
| Reconstitution and Storage | -20°C|2°C to 8°C | ||||||||||||||||||||||
| Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 1.56 ng/mL | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | Mmp-13 |
|---|---|
| Alias Symbols | MMP-13 |
| NCBI Gene Id | 395683 |
| Protein Name | Submitted name: Matrix metalloproteinase-13 |
| Uniprot ID | O93363 |
| Protein Accession # | NP_001280019 |
| Nucleotide Accession # | NM_001293090 |
