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> mouse Anti-COX 2 (Cyclooxygenase 2, PGHS 2, Prostaglandin Endoperoxide Synthase 2, PHS 2) (FITC) Monoclonal Antibody [Clone:6D319 (CX229)]

mouse Anti-COX 2 (Cyclooxygenase 2, PGHS 2, Prostaglandin Endoperoxide Synthase 2, PHS 2) (FITC) Monoclonal Antibody [Clone:6D319 (CX229)]

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Référence C7904-82K-1ea

Conditionnement : 1ea

Marque : US Biological

COX 2 (Cyclooxygenase 2, PGHS 2, Prostaglandin Endoperoxide Synthase 2, PHS 2) (FITC)

C7904-82K COX 2 (Cyclooxygenase 2, PGHS 2, Prostaglandin Endoperoxide Synthase 2, PHS 2) (FITC)

Clone Type
Monoclonal
Host
mouse
Source
human
Swiss Prot
P35354
Isotype
IgG1
Grade
Purified
Applications
FC IC
Crossreactivity
Hu Sh
Shipping Temp
Blue Ice
Storage Temp
-20°C

Cyclooxygenase catalyzes the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins: the conversion of arachidonic acid to prostaglandin H2. Recent discoveries of the induction of cyclooxygenase biosynthesis by a variety of stimuli such as phorbol esters, lipopolysaccharides, and cytokines led to the hypothesis that the inducible form of cyclooxygenase, COX-2, is responsible for the biosynthesis of prostaglandins under acute inflammatory conditions. COX-2 is a 72kD protein which has been cloned from a variety of species including human, mouse, rat, and sheep.

Applications:
Suitable for use in Flow Cytometry and Immunocytochemistry. Not recommended for Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1ug/10e6 cells
Immunocytochemistry: 2-5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 6D319 (CX229)|Host: mouse|Source: human|Concentration: ~0.2mg/ml|Form: Supplied as a liquid in PBS, pH 7.2, 4mg/ml BSA, 0.02% sodium azide, 50% glycerol. Labeled with fluorescein isothiocyanate (FITC).|Purity: Purified|Immunogen: Synthetic peptide corresponding to aa580-599, NASSSRSGLDDINPTVLLKE of human COX-2. Species sequence homology: bovine and canine.|Specificity: Recognizes human COX-2. Species crossreactivity: sheep. |Does not cross-react with mouse, rat, and rabbit COX-2 or COX-1 (all species). ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Synthetic peptide corresponding to aa580-599, NASSSRSGLDDINPTVLLKE of human COX-2. Species sequence homology: bovine and canine.
Form
Supplied as a liquid in PBS, pH 7.2, 4mg/ml BSA, 0.02% sodium azide, 50% glycerol. Labeled with fluorescein isothiocyanate (FITC).
Purity
Purified
Specificity
Recognizes human COX-2. Species crossreactivity: sheep. |Does not cross-react with mouse, rat, and rabbit COX-2 or COX-1 (all species).
References
1.Hla, T., Neilson, K.. Human cyclooxygenase-2 cDNA. Proc Natl Acad Sci USA 89 7384-7388 (1992).2. Xie, W., Chipman, J.G., Robertson, D.L., et al. Expression of a mitogen-responsive gene encoding prostaglandin synthase is regulated by mRNA splicing. Proc Natl Acad Sci USA 88 2692-2696 (1991).3. Kujubu, D.A., Fletcher, B.S., Varnum, B.C., et al. TIS10, a phorbol ester tumor promoter-inducible mRNA from Swiss 3T3 cells, encodes a novel prostaglandin synthase/cyclooxygenase homologue. J Biol Chem 266 12866-12872 (1991).4. Yamagata, K., Andreasson, K.I., Kaufmann, W.E., et al. Expression of a mitogen-inducible cyclooxygenase in brain neurons: Regulation by synaptic activity and glucocorticoids. Neuron 11 371-386 (1993).5. Zhang, V., O'Sullivan, M., Hussain, H., et al. Molecular cloning, functional expression, and selective regulation of ovine prostaglandin H synthase-2. Biochem Biophys Res Commun 227 499-506 (1996).6. Litherland, S.A., Xie, X.T., Hutson, A.D., et al. Aberrant prostaglandin synthase 2 expression defines an antigen-presenting cell defect for insulin-dependent diabetes mellitus. J Clin Invest 104 515-523 (1999).