Mouse Anti-FLAG Antibody
Référence OAPA00047
Conditionnement : 0.5mg
Marque : Aviva Systems Biology
Mouse Anti-FLAG Antibody (OAPA00047)
| Datasheets/Manuals | Printable datasheet for Mouse Anti-FLAG Antibody (OAPA00047) |
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| Product Format | Lyophilized PBS Physical Appearance: Sterile filtered white freeze-dried powder |
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| Clonality | Polyclonal |
| Clone | NYRFLAG |
| Isotype | IgG1, kappa. |
| Host | Mouse |
| Reconstitution and Storage | Reconstitute with H20 to get a 1 mg/ml concentration. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. In lyophilized form, for long periods, store at 4C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20C. |
| Immunogen | Flag-conjugated proteins. |
| Purification | Protein A & Ion exchange column. |
| Specificity | Will recognize FLAG (DYKDDDDK) conjugated at either the amino or carboxy terminus. Can be used bound to various resins for affinity purifications. |
| Source | BALB/c mice |
| Application Info | The FLAG peptide purifies and detects recombinant fusion proteins. FLAG peptide is useful in Western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization. Flag peptide is a hydrophilic tag which significantly improves the detection and purification of recombinant fusion proteins. Titer: By direct ELISA, 1:20,000 dilution will yield >1.0 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories). |
| Gene Symbol | RGS3 |
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| Description of Target | A fusion tag called FLAG consists of eight amino acids (AspTyrLysAspAspAspAspLys) including an enterokinase-cleavage site. FLAG is specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody. denoted as M1. binds the peptide in the presence of bivalent metal cations. preferably Ca(+). Elution is effected by chelating agents. Another strategy is competitive elution with excess of free Flag Peptide. The FLAG peptide purifies and detects recombinant fusion proteins. FLAG peptide is useful in Western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization. Flag peptide is a hydrophilic tag which significantly improves the detection and purification of recombinant fusion proteins. |
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What is the species homology for "Mouse Anti-FLAG Antibody (OAPA00047)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "".
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How long will it take to receive "Mouse Anti-FLAG Antibody (OAPA00047)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "Mouse Anti-FLAG Antibody (OAPA00047)" provided in?
This item is provided in "Lyophilized PBS
Physical Appearance: Sterile filtered white freeze-dried powder".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Mouse Anti-FLAG Antibody (OAPA00047)"?
This target may also be called "" in publications.
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What is the shipping cost for "Mouse Anti-FLAG Antibody (OAPA00047)"?
The shipping cost for this item is within the US. Please contact us for specific shipping for international orders.
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What is the guarantee for "Mouse Anti-FLAG Antibody (OAPA00047)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Mouse Anti-FLAG Antibody (OAPA00047)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Mouse Anti-FLAG Antibody (OAPA00047)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "RGS3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "RGS3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "RGS3"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "RGS3"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "RGS3"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "RGS3"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.