General Information
| Short Description | This IL6R Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma, tissue homogenates or other biological fluids.. |
| Applications | ELISA |
| Reactivity | Mouse |
| Sensitivity | 11.4pg/mL |
| Detection Limit | 31.2-2000pg/mL |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Storage Instruction | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time | 4.5 hrs |
Target Information
| Gene Symbol | |
| Gene ID | |
| Uniprot ID | |
| Sample Type | serum, plasma, tissue homogenates or other biological fluids. |
Additional Info
| Tissue Specificity | Expressed by dendritic cells. Soluble interleukin-6 receptor subunit alpha: Detected in the cerebrospinal fluid. |
| Post Translational Modifications | A short soluble form is also released from the membrane by proteolysis. The sIL6R is formed by limited proteolysis of membrane-bound receptors, a process referred to as ectodomain shedding. mIL6R is cleaved by the proteases ADAM10 and ADAM17. Glycosylated. Glycosylation is dispensable for transport, signaling, and cell-surface turnover. Glycosylation at Asn-55 is a protease-regulatory exosite. Glycosylation is required for ADAM17-mediated proteolysis. |
| Function | Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation leads to the regulation of the immune response, acute-phase reactions and hematopoiesis. The interaction with membrane-bound IL6R and IL6ST stimulates 'classic signaling', the restricted expression of the IL6R limits classic IL6 signaling to only a few tissues such as the liver and some cells of the immune system. Whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells. Interleukin-6 receptor subunit alpha: Signaling via the membrane-bound IL6R is mostly regenerative and anti-inflammatory (Probable). Drives naive CD4(+) T cells to the Th17 lineage, through 'cluster signaling' by dendritic cells. Soluble interleukin-6 receptor subunit alpha: Soluble form of IL6 receptor (sIL6R) that acts as an agonist of IL6 activity. The IL6:sIL6R complex (hyper-IL6) binds to IL6ST/gp130 on cell surfaces and induces signaling also on cells that do not express membrane-bound IL6R in a process called IL6 'trans-signaling'. sIL6R is causative for the pro-inflammatory properties of IL6 and an important player in the development of chronic inflammatory diseases. In complex with IL6, is required for induction of VEGF production. Plays a protective role during liver injury, being required for maintenance of tissue regeneration. 'Trans-signaling' in central nervous system regulates energy and glucose homeostasis. |
| Protein Name | Interleukin-6 Receptor Subunit AlphaIl-6 Receptor Subunit AlphaIl-6r Subunit AlphaIl-6r-AlphaIl-6raIl-6r 1Cd Antigen Cd126 Cleaved Into - Soluble Interleukin-6 Receptor Subunit AlphaSil6r |
| Database Links | |
| Cellular Localisation | Interleukin-6 Receptor Subunit Alpha: Cell MembraneSingle-Pass Type I Membrane ProteinSoluble Interleukin-6 Receptor Subunit Alpha: Secreted |
| Alternative ELISA Names | Interleukin-6 Receptor Subunit Alpha ELISA kitIl-6 Receptor Subunit Alpha ELISA kitIl-6r Subunit Alpha ELISA kitIl-6r-Alpha ELISA kitIl-6ra ELISA kitIl-6r 1 ELISA kitCd Antigen Cd126 Cleaved Into - Soluble Interleukin-6 Receptor Subunit Alpha ELISA kitSil6r ELISA kitIl6ra ELISA kitIl6r ELISA kit |
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Information sourced from Uniprot.org