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> MRAS (NM_012219) Human Untagged Clone

MRAS (NM_012219) Human Untagged Clone

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Référence SC115494

Conditionnement : 10ug


MRAS (NM_012219) Human Untagged Clone

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SKU
SC115494
MRAS (untagged)-Human muscle RAS oncogene homolog (MRAS), transcript variant 1
 

Other products for "MRAS or NM_012219"

Specifications
Product Data
Type Human Untagged Clone
Target Symbol MRAS
Synonyms M-RAs; NS11; R-RAS3; RRAS3
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF sequence for NM_012219 edited
ATGGCAACCAGCGCCGTCCCCAGTGACAACCTCCCCACATACAAGCTGGTGGTGGTGGGG
GATGGGGGTGTGGGCAAAAGTGCCCTCACCATCCAGTTTTTCCAGAAGATCTTTGTGCCT
GACTATGACCCCACCATTGAAGACTCCTACCTGAAACATACGGAGATTGACAATCAATGG
GCCATCTTGGACGTTCTGGACACAGCTGGGCAGGAGGAATTCAGCGCCATGCGGGAGCAA
TACATGCGCACGGGGGATGGCTTCCTCATCGTCTACTCCGTCACTGACAAGGCCAGCTTT
GAGCACGTGGACCGCTTCCACCAGCTTATCCTGCGCGTCAAAGACAGGGAGTCATTCCCG
ATGATCCTCGTGGCCAACAAGGTCGATTTGATGCACTTGAGGAAGATCACCAGGGAGCAA
GGAAAAGAAATGGCGACCAAACACAATACTCCGTACATAGAAACCAGTGCCAAGGACCCA
CCTCTCAATGTCGACAAAGCCTTCCATGACCTCGTTAGAGTAATTAGGCAACAGATTCCG
GAAAAAAGCCAGAAGAAGAAGAAGAAAACCAAATGGCGGGGAGACCGGGCCACAGGCACC
CACAAACTGCAATGTGTGATCTTGTGA
5' Read Nucleotide Sequence
>OriGene 5' read for NM_012219 unedited
GCGAATTCGGCACGAGGGCCGTCAGTCCGGCGGCCGCGGGGCCCGGCGGGCGCGACGCTG
CCTCCTCACCGGCGCAGGCTAGGAGGGGGCGGCCTGAGTGCCGTAGCCGAGCCGGGGCTG
GAGCGCGCGGTCTGACCTACGAGAAACATGGCAACCAGCGCCGTCCCCAGTGACAACCTC
CCCACATACAAGCTGGTGGTGGTGGGGGATGGGGGTGTGNACAAAACATCCCTCACCATC
CACTTTTCTCTCAATACTTTTGTTCCATGTTTTGACCTCCCATTTGCAAACTTGTACGTG
ATACATCAGCTTATTGTCATCTACTGCCTTATCTAGCTCTTACTGCTTACCAATTGCTTC
GAGGAATTAACTTCCTTCCAGCATCAATTACCTTTCTCTTTAAACTGTCTTCCTAATCGT
CCACTACGGTCCTGAAAATTGCAACTTTTAGACATCTTCGACACGTTTCCCAAAGCAAAC
CCTGCACCCGCCAATCTCCTGCAGCTACTACCGGTCTGACACATTTACTCCTCTTTGCCT
TCTTCCTTGTTCTTCATCCACATCACCTGCGCAGCCTTTCTTTTTCATCTCACATCTATC
CCTTTCCACTCTGCTTCACTCCACTCTATGTGCCCAGTCACCCGCATTGTCAACCGTTCG
AAATACCATCCTCGCTGAACGTCCTCTAAACACTCTTATTCCTCCACCATTTCGACACAC
AATCCCCTTATTCAACATCCATATCAACCCCTAATGCCGGCCCCAACCTCTGTCAACTTT
CCTACCCCAACACTCCTACCAGAAAATATCCTCCTCCCAGCCCAT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_012219 unedited
CTAAGGACCGCGGCCGCATCTANGATCGGTTTTTTTTTTTTTTTTTTTACGTGTTTCTCT
TCTTTATTAATCAGTCGAGAGGAACAAAGACTTAATAAAAAGTTACACAGAAGGTTCAAA
CATCACTGAGATCTTCAGGCATCACTCGTTTCACGACAATGGCCACCTGTTAAGACTATG
AAACAGAAGCTGGGCGCTCACAGGCGCACAATAATTCAAGTATCTTTCATTCCAAGTAAA
CCCGACATGGAATTTCAAACACAATGGGCTGCCAATGGAGGGAACCTGTCTGTGGCTCCA
GATTTGCTGTAACATGTCTGAGGGGCCTTTGGGTTAAAGTTGCCACGTTAGTGGGTCATG
TCTCCTGGAAAAGTGAGACAGGCCCCACGAGGCCAGCCAGGCATTTTCCACAGCAAGTGC
CATGAGAAGGAGGTGGGCAAAGTCCTAAGAGAAACCATCCAGGTGTCCCGAGTGGCAAAG
TGCACAGGTAGTAAGTGGCCATTGTCCCCAGCCGTGGGAGTGGAGAGGATGAGTTGGCTT
ACCTCCGGATAAAAAAGAGCGCACTTGTGAGGAAGTATCAAAGGGCGGTACAGCCCAGAA
CCTAGAAAAGGGGAGAAGAGATGACGAGGGGGCATGTCGGGGAGGGGAAGGAAAGGAGGA
CGCAGAAAATGGCGGGGAGAAACGGAAGCACCACGTAATGGAAAGAGAGCGGGCGTACCA
TCACAGAGGAAATAGGGGAGCGGAACTACACGCAAAAAAAAAGGAGACCAGTCGAACGGG
CAAGATATACACAGAGGGCGAAGGCGCACCGACGGCTGCCGCCAAGATGCAAAAGAGGCA
AAGAGAGAAACCACCGGAGGCCTATGTTAAGAGAGAAAGGATACAGCGAGTTCCAGACAA
GAGAGGGTAAGGATAAGAGGGCCAAACATAAGGAAAGGACACAACAGACGGCATAGACAG
AAAAGGCAATATAAGCCGACCAGAATGAGAGAAGTCAAG
Restriction Sites NotI-NotI |
ACCN NM_012219
Insert Size 4700 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012219.2, NP_036351.2
RefSeq Size 3926 bp
RefSeq ORF 627 bp
Locus ID 22808
UniProt ID O14807
Cytogenetics 3q22.3
Domains RAB, RAN, ras, RAS, RHO
Protein Families Druggable Genome
Protein Pathways MAPK signaling pathway, Regulation of actin cytoskeleton, Tight junction
Summary This gene encodes a member of the Ras family of small GTPases. These membrane-associated proteins function as signal transducers in multiple processes including cell growth and differentiation, and dysregulation of Ras signaling has been associated with many types of cancer. The encoded protein may play a role in the tumor necrosis factor-alpha and MAP kinase signaling pathways. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. provided by RefSeq, Nov 2011
Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same isoform (1).
SKU Description Size
RC212259 MRAS (Myc-DDK-tagged)-Human muscle RAS oncogene homolog (MRAS), transcript variant 1 10 ug
RC212259L3 Lenti-ORF clone of MRAS (Myc-DDK-tagged)-Human muscle RAS oncogene homolog (MRAS), transcript variant 1 10 ug
RC212259L4 Lenti-ORF clone of MRAS (mGFP-tagged)-Human muscle RAS oncogene homolog (MRAS), transcript variant 1 10 ug
RG212259 MRAS (tGFP-tagged) - Human muscle RAS oncogene homolog (MRAS), transcript variant 1 10 ug

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