Preparation of MyoUp medium

The MyoUp medium for cultivation of LHCN-M2 cells can either be ordered from Evercyte as ready-to-use medium (Cat#MHT-040) or as basal medium (MHT-050-B) + Supplements (MHT-040-S) or can be prepared by mixing the following components:

take one bottle (500 ml) of DMEM (Gibco, Cat # 61965-026) and discard 100 ml

add 10 ml Hepes (1M stock, ready-to-use, Sigma-Aldrich, Cat# H0887)

add 50 µl Zink Sulfate stock (30 mg/ml, prepared in cell culture grade water, Sigma-Aldrich, Cat# Z0251)

add 50 µl Vitamin B12 stock (14 mg/ml, prepared in cell culture grade water, Sigma-Aldrich, Cat# V2876)

add 70 µl Dexamethasone stock (1 mM, prepared in absolute ethanol/DMEM, Sigma-Aldrich, Cat# D4902)

add 25 µl HGF stock (50 µg/ml, prepared in cell culture grade water, Merck Millipore, Cat# GF116)

add 50 µl bFGF stock solution (50 µg/ml, prepared in cell culture grade water, Enantis, Cat# FGF-STAB)

mix properly and store at 4°C for up to 1 month

Storage conditions

Store the basal medium at 4°C for up to 6 months and the supplements -20°C for up to 6 months.

Protocols