NeoLume™ ECL Substrate – Attogram Sensitivity
Description
NeoLume Attogram Sensitivity is an ultra-sensitive chemiluminescent (ECL) substrate for horseradish peroxidase (HRP)-based immunoassays. Its proprietary technology combines extreme sensitivity with exceptionally high light output, enabling detection of trace amounts of proteins at the attogram level.
The formulation provides low background and an extremely high signal-to-noise ratio, supporting accurate CLIA analysis and precise interpretation of very low analyte concentrations. It is particularly suited to limited samples and proteins that are difficult to detect.
Compared with conventional colorimetric methods, chemiluminescent immunoassays offer greater sensitivity, faster protocols, and lower reagent consumption.
NeoLume Attogram Sensitivity can be used in high-throughput enzymatic immunoassays measured with a microplate chemiluminescence reader. Its strong, immediate light output reaches maximum signal intensity within 1 minute after substrate addition.
The substrate enables the use of very low antigen and antibody concentrations while maintaining exceptional signal intensity and low background.
Key Benefits
- Attogram-level detection: sensitivity for trace protein quantities and very low analyte concentrations.
- High signal-to-noise ratio: strong light output with low background for precise interpretation.
- Rapid signal generation: maximum signal intensity within 1 minute of substrate addition.
- Reduced assay time and reagent consumption: suitable for low antigen and antibody concentrations.
- High lot-to-lot consistency.
- High-throughput compatibility: enzymatic immunoassays measured with microplate chemiluminescence readers.
Product Properties
| Property | Details |
|---|---|
| Features | Attogram detection level. Maximized sensitivity. Reduced assay time and consumption of precious reagents. High lot-to-lot consistency. |
| Components | Luminol derivative/enhancer solution (A). Peroxide solution (B). |
| Antibody dilution range | Primary: 1:10,000–1:200,000. Secondary: 1:300,000–1:1,000,000. (From 1 mg/mL stock solutions.) |
| Available formats | 2 × 10 mL kit (200 cm2 of membrane). 2 × 50 mL kit (1,000 cm2 of membrane). |
| Signal duration | 5 hours. |
| Storage | Stable for 1 year at room temperature. |
| Shipping | Ambient temperature. |