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Neomycin sulfate [1405-10-3]

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Référence NB-64-01112-5g

Conditionnement : 5g

Marque : Neo Biotech

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Neomycin sulfate

(Synonyms: Neomycin sulphate, Framycin sulfate) Copy Product Info

Synonyms: Neomycin sulphate, Framycin sulfate

Catalog No. T0950 Copy Product Info

Purity: 99.88%

Neomycin sulfate (Framycin sulfate) is a broad-spectrum aminoglycoside antibiotic that blocks the synthesis of bacterial proteins in order to exert antimicrobial activity. Neomycin sulfate is commonly used to screen prokaryotic and eukaryotic cells for Neo resistance genes.

Cas No. 1405-10-3

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.88%

Color:White to Yellow

Product Introduction

Bioactivity

Description	Neomycin sulfate (Framycin sulfate) is a broad-spectrum aminoglycoside antibiotic that blocks the synthesis of bacterial proteins in order to exert antimicrobial activity. Neomycin sulfate is commonly used to screen prokaryotic and eukaryotic cells for Neo resistance genes.
Targets & IC50	ATP-induced current:90 μM, PC-PLD:65 µM
In vitro	METHODS: BHK-21 cells, VERO cells and FEA cells were treated with Neomycin sulfate (1000-20000 μg/mL) for 24 h. Cell viability was measured by MTT. RESULTS: The viability of BHK-21 cells was significantly reduced by 9000-20000 μg/mL Neomycin treatment. 3000 μg/mL Neomycin treatment significantly reduced the viability of FEA cells. 20000 μg/mL Neomycin treatment did not affect the viability of VERO cells. [1] METHODS: Mouse cochlear hair cells HEI-OC-1 were treated with Neomycin sulfate (2 mM) for 24 h. Apoptosis was detected by Flow Cytometry. RESULTS: 20 μM GM1 significantly reduced Neomycin sulfate-induced cell death in HEI-OC-1 cells. [2]
In vivo	METHODS: To assay in vivo activity, Neomycin sulfate (20 mg/mouse) and bacitracin (20 mg/mouse) were administered by gavage to C57BL/10 mice once daily for seven days. RESULTS: The combination of Neomycin and bacitracin decreased intestinal permeability and increased gene expression of ZO-1, JAM-A, and occludin in the ileum and ZO-1, claudin-3, and claudin-4 in the colon. [3] METHODS: To deplete the intestinal microbiota of mice, the antibiotics (ABX) vancomycin (0.5 g/L), ampicillin (1 g/L), Neomycin sulfate (1 g/L), and metronidazole (1 g/L) were administered to mice by drinking water for two weeks. RESULTS: Antibiotics significantly reduced the diversity and composition of the gut microbiota. [4]
Synonyms	Neomycin sulphate, Framycin sulfate

Chemical Properties

Molecular Weight	908.87
Formula	C₂₃H₄₆N₆O₁₃·3H₂SO₄
Cas No.	1405-10-3
Smiles	OS(O)(=O)=O.OS(O)(=O)=O.OS(O)(=O)=O.NC[C@@H]1O[C@H](O[C@@H]2[C@@H](CO)O[C@@H](O[C@@H]3[C@@H](O)[C@H](N)C[C@H](N)[C@H]3O[C@H]3O[C@H](CN)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O)[C@H](N)[C@@H](O)[C@@H]1O
Relative Density.	no data available

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: Insoluble

H2O: 460 mg/mL (506.12 mM)

In Vivo Formulation

PBS: 100 mg/mL (110.03 mM)

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O

	1mg	5mg	10mg	50mg
1 mM	1.1003 mL	5.5013 mL	11.0027 mL	55.0134 mL
5 mM	0.2201 mL	1.1003 mL	2.2005 mL	11.0027 mL
10 mM	0.1100 mL	0.5501 mL	1.1003 mL	5.5013 mL
20 mM	0.0550 mL	0.2751 mL	0.5501 mL	2.7507 mL
50 mM	0.0220 mL	0.1100 mL	0.2201 mL	1.1003 mL
100 mM	0.0110 mL	0.0550 mL	0.1100 mL	0.5501 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

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Keywords

synthesisribosomalproteinproliferationPhospholipaseNeomycin sulfateNeomycinInhibitorinhibitFramycin SulfateBacterialAntibioticangiogeninaminoglycoside30S ribosome

Related Tags: Neomycin sulfate chemical structure | Neomycin sulfate in vivo | Neomycin sulfate in vitro | Neomycin sulfate formula | Neomycin sulfate molecular weight