Normal Rat Serum (Filtered)

Store at 20°C or 80°C.

This product contains no preservatives or anticoagulants.

Shipped on Dry Ice. Upon delivery aliquot and store at 20°C or 80°C. Avoid repeated freeze/thaw cycles.

Normal rat serum is used in research for preventing unwanted antibody interactions during tissue and cell staining experiments.

It blocks two main issues:
1) Fc receptor binding The serum blocks Fc receptors on the sample from attaching to the primary and secondary antibodies used in the experiment.
2) Nonspecific antibody binding It also prevents the antibodies themselves from sticking to irrelevant parts of the sample.

What to look for when choosing a blocking serum:
1) Species matters The serum typically comes from a different animal species than the one used to create the primary antibody.
2) Secondary antibody consideration Often, the blocking serum is derived from the same species as the secondary antibody.

This serum can be used in two ways:
1) Direct application The serum is applied directly to the sample for blocking.
2) Blocking buffer component The serum can be incorporated into a solution specifically designed for blocking, called a blocking buffer.

Normal serum diluted to 5% (v/v) in PBS (or comparable buffer) is strongly recommended as a blocking agent to reduce background from nonspecific, conservedsequence, and/or Fcreceptor binding.