Conditionnement : 60rxns
The pEASY®-T1 Cloning Vector contains the LacZ gene, enabling blue-white screening on plate media with IPTG and X-gal, making it suitable for TA cloning.
Features
Fast: Takes only 5 minutes.
Simple: Just add the fragment.
Efficient: High positive rate.
Provides two selection markers, ampicillin and kanamycin, allowing flexible selection based on experimental needs.
Multiple restriction enzyme sites for convenient subcloning.
Sequencing primers: M13 Forward Primer, M13 Reverse Primer, T7 Promoter Primer.
T7 Promoter for in vitro transcription.
Trans1-T1 competent cells offer high transformation efficiency and fast growth, ensuring high clone yield and reducing screening time.
Storage
Trans1-T1 Phage Resistant Chemically Competent Cell at -70℃ or below for one year; others at -18℃ or below for one year.
Shipping
Dry ice (-70℃)
| Component | CT101-01 (20 rxns) | CT101-02 (60 rxns) |
| pEASY ®-T1 Cloning Vector (10 ng/μl) | 20 μl | 3×20 μl |
| Control Template (5 ng/μl) | 5 μl | 5 μl |
| Control Primers (10 μM) | 5 μl | 5 μl |
| M13 Forward Primer (10 μM) | 50 μl | 150 μl |
| M13 Reverse Primer (10 μM) | 50 μl | 150 μl |
| Trans1-T1 Phage Resistant Chemically Competent Cells | 10×100 μl | 30×100 μl |
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7 Liu Q, Deng S, Liu B, et al. A helitron-induced RabGDIα variant causes quantitative recessive resistance to maize rough dwarf disease[J]. Nature Communications, 2020.(IF 12.12)
Manual