Pepsin Reagent (ready to use)
Référence MBS258489-15mL
Conditionnement : 15mL
Marque : MyBiosource
Pepsin Reagent – ready to use
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS258489Pepsin is an antigen retriever solution used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and… More details
Product Overview
Online Datasheet in PDFProduct Name
Pepsin, Reagent
Full Product Name
Pepsin Reagent (ready to use)
Preparation and Storage
Store at 2-8 degree C. DO NOT FREEZE
Notes
Ready-to use Pepsin reagent
Product Categories/Family for Pepsin reagent
IHC Reagents
Applications Tested/Suitable for Pepsin reagent
Immunohistochemistry (IHC)
Application Notes for Pepsin reagent
1. Deparafinize FFPE tissue section is carried out as outlined in your protocol. Hydrate tissue with PBS or Universal Immuno buffer.
2. Remove buffer, add 2-4 drops of Pepsin reagent to cover tissue section.
3. Incubate at 37 degree C or at 56 degree C for 5-10 minutes (the time required for optimum digestion of FFPE tissue section will vary with the extent of fixation, generally approx. 5-10 minutes are sufficient at the above temperatures. In some cases it may require 15 minute.).
4. Discard Pepsin reagent, wash 3-5 times with buffer.
5. The tissue sections are ready for further IHC protocol. NOT RECOMMENDED FOR CYTOKERATIN (clone: AE3) ANTIBODY
2. Remove buffer, add 2-4 drops of Pepsin reagent to cover tissue section.
3. Incubate at 37 degree C or at 56 degree C for 5-10 minutes (the time required for optimum digestion of FFPE tissue section will vary with the extent of fixation, generally approx. 5-10 minutes are sufficient at the above temperatures. In some cases it may require 15 minute.).
4. Discard Pepsin reagent, wash 3-5 times with buffer.
5. The tissue sections are ready for further IHC protocol. NOT RECOMMENDED FOR CYTOKERATIN (clone: AE3) ANTIBODY
Other Notes
Small volumes of Pepsin reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for Pepsin purchase
MBS258489 is a reagent targeting Pepsin. Also known as Pepsin Reagent (ready to use).
Additional Details
NCBI/Uniprot data below describe general gene information for Pepsin. It may not necessarily be applicable to this product.
Protein Family
Pepsin
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.