Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.

POPG (sodium salt) [268550-95-4]

Imprimer

Référence HY-130463-5mg

Conditionnement : 5mg

Marque : MedChemExpress

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Description

POPG (sodium) is a negatively charged phospholipid. POPG (sodium) affects the interactions of membrane proteins with other molecules by changing the charge characteristics of the lipid environment. POPG (sodium) increases the apparent affinity of Gαq and Gβ₁γ₁ for activated NTS1. POPG (sodium) can interact with the positive charge of peptides. POPG sodium salt can be used in Parkinson's disease research^[1]^[2]^[3]^[4].

In Vitro

POPG (sodium) can be used in the preparation of nanoparticles capable of co-encapsulating and releasing hydrophilic peptide compounds^[1].
POPG (sodium) increases the apparent affinity of Gαq and Gβ₁γ₁ for activated NTS1^[2].
POPG (sodium)-formed bilayers can be penetrated by the antimicrobial peptide LL-37^[3].
The presence of POPG (sodium) lipids in the membrane enhances the binding of the PAP248-286 peptide^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Solvant et solubilité

In Vitro:

DMSO : 18.18 mg/mL (23.58 mM; ultrasonic and adjust pH to 4 with 1 M HCl; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 8.33 mg/mL (10.80 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.2970 mL	6.4852 mL	12.9703 mL
5 mM	0.2594 mL	1.2970 mL	2.5941 mL
10 mM	0.1297 mL	0.6485 mL	1.2970 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (1.62 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 1.25 mg/mL (1.62 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 1.25 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.83 mg/mL (1.08 mM); Clear solution

This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (8.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 4

Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 0.83 mg/mL (1.08 mM); Clear solution

This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (8.3 mg/mL) to 900 μL Corn oil, and mix evenly.

Pureté et documentation

Purity: 99.23%

Fiche technique (268 KB) SDS (647 KB)

COA (240 KB) HNMR (293 KB) RP-HPLC (249 KB) MS (85 KB)

Instruction de manipulation (2659 KB)

Références

[1]. Kim MR, et al. Co-Encapsulation and Co-Delivery of Peptide Drugs via Polymeric Nanoparticles. Polymers (Basel). 2019;11(2):288. Published 2019 Feb 8. [Content Brief]

[2]. Inagaki S, et al. Modulation of the interaction between neurotensin receptor NTS1 and Gq protein by lipid. J Mol Biol. 2012 Mar 16;417(1-2):95-111. [Content Brief]

[3]. Zhao L, et al. Molecular Dynamics Simulations of Human Antimicrobial Peptide LL-37 in Model POPC and POPG Lipid Bilayers. Int J Mol Sci. 2018 Apr 13;19(4):1186. [Content Brief]

[4]. Agrawal N, et al. Investigating the Effects of the POPC-POPG Lipid Bilayer Composition on PAP248-286 Binding Using CG Molecular Dynamics Simulations. J Phys Chem B. 2023 Oct 26;127(42):9095-9101. [Content Brief]

POPG (sodium salt) [268550-95-4]

Référence HY-130463-5mg

Marque : MedChemExpress

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