Orb1310235-1ml | Pregnenolone [145-13-1] Clinisciences

Pregnenolone

SKU: orb1310235

Description

Pregnenolone

Research Area

Cell Biology, Metabolism Research, Neuroscience, Pharmacology & Drug Discovery

Images & Validation

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Key Properties

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CAS Number	145-13-1
MW	316.4776
Purity	>99.99% (May vary between batches)
Formula	C₂₁H₃₂O₂
SMILES	CC(=O)C1CCC2C3CC=C4CC(O)CCC4(C)C3CCC12C
Target	TRP/TRPV Channel,Cannabinoid Receptor,Endogenous Metabolite,AChR,Autophagy
Solubility	Ethanol:21 mg/mL (66.35 mM);DMSO:3.33 mg/mL (10.52 mM);H2O:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

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In Vivo	Pregnenolone sustains its proliferative activity in vivo and stimulates the growth of LNCaP-tumor xenografts in intact male SCID mice as well as in castrated animals. Pregnenolone is shown to activate transcription through the LNCaP androgen receptor (AR), but not the wild-type AR. Pregnenolone results in a significant decrease in the accumulation of astrocytes in the proximity of the wound and in a decreased bromodeoxyuridine incorporation in reactive astrocytes of rats. Pregnenolone administration results in elevations in downstream neurosteroids such as allopregnanolone, a molecule with neuroprotective effects that also increases neurogenesis, decreases apoptosis and inflammation, modulates the hypothalamic-pituitary-adrenal axis, and markedly increases GABA(A) receptor responses. Pregnenolone administration elevates pregnenolone sulfate, a neurosteroid that positively modulates NMDA receptors.
In Vitro	Pregnenolone induces a large, dose-related increase of both the rate and extent of MAP2-induced tubulin assembly, whereas progesterone, inactive per se, counteracted the stimulatory effect of Pregnenolone. Pregnenolone-increased assembly of microtubules produces a completely normal structure. Pregnenolone preserves microtubule abundance and promotes cell movement during epiboly. Pregnenolone results in dramatic reduction in GR nuclear localization in mouse hippocampal cell line (HT-22). Pregnenolone has neuroprotective effects against both glutamate and amyloid beta protein neuropathology.

In Vivo

Pregnenolone sustains its proliferative activity in vivo and stimulates the growth of LNCaP-tumor xenografts in intact male SCID mice as well as in castrated animals. Pregnenolone is shown to activate transcription through the LNCaP androgen receptor (AR), but not the wild-type AR. Pregnenolone results in a significant decrease in the accumulation of astrocytes in the proximity of the wound and in a decreased bromodeoxyuridine incorporation in reactive astrocytes of rats. Pregnenolone administration results in elevations in downstream neurosteroids such as allopregnanolone, a molecule with neuroprotective effects that also increases neurogenesis, decreases apoptosis and inflammation, modulates the hypothalamic-pituitary-adrenal axis, and markedly increases GABA(A) receptor responses. Pregnenolone administration elevates pregnenolone sulfate, a neurosteroid that positively modulates NMDA receptors.

In Vitro

Pregnenolone induces a large, dose-related increase of both the rate and extent of MAP2-induced tubulin assembly, whereas progesterone, inactive per se, counteracted the stimulatory effect of Pregnenolone. Pregnenolone-increased assembly of microtubules produces a completely normal structure. Pregnenolone preserves microtubule abundance and promotes cell movement during epiboly. Pregnenolone results in dramatic reduction in GR nuclear localization in mouse hippocampal cell line (HT-22). Pregnenolone has neuroprotective effects against both glutamate and amyloid beta protein neuropathology.

Storage & Handling

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Storage	-20°C
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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EndogenousMetabolite, 3β-Hydroxy-5-pregnen-20-one, 3b-Hydroxy-5-pregnen-20-one, 3beta-Hydroxy-5-pregnen-20-one, CB, CannabinoidReceptor, Cannabinoid Receptor, Autophagy, Arthenolone, Inhibitor, Endogenous Metabolite, inhibit, mAChR, Pregnenolone, Transient receptor potential channels, TRPChannel, TRP Channel, TRPVChannel, TRPV Channel

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Human Pregnenolone (PREG) ELISA Kit [orb866912]
Reactivity:
Human
Dynamic Range:
15.63-1000 pg/mL
Sensitivity:
5.25 pg/mL
Sizes Available: 48 T, 96 T
General Pregnenolone (PREG) ELISA Kit [orb1806503]
Reactivity:
All
Dynamic Range:
0.78-50ng/mL
Sensitivity:
0.47 ng/mL
Sizes Available: 48 T, 96 T
General Pregnenolone (PREG) ELISA Kit [orb2307999]
Reactivity:
All
Dynamic Range:
15.63-1000 pg/mL
Sensitivity:
5.88 pg/mL
Sizes Available: 96 T, 48 T
Rat Pregnenolone (PREG) ELISA Kit [orb2307989]
Reactivity:
Rat
Dynamic Range:
15.63-1000 pg/mL
Sensitivity:
6.48 pg/mL
Sizes Available: 96 T, 48 T
StAR Antibody / Steroidogenic acute regulatory protein [orb606624]
Applications:
IHC-P
Reactivity:
Human
Species/Host:
Mouse
Clonality:
Monoclonal
Conjugation:
Unconjugated
Sizes Available: 100 μg, 20 μg