Rabbit Anti-LC3 (APG8A) (N-term) Polyclonal Antibody
Référence AP1801A
Conditionnement : 400ul
Marque : Abcepta
LC3 Antibody (APG8A) (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
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Fluorescent image of U251 cells stained with AP1801a LC3 (APG8A) (N-term) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1801a LC3 (APG8A) (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
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Western blot analysis of anti-LC3 (APG8a) Pab (Cat. #AP1801a) in rat brain lysate. Both lipidated (arrow, II) and non-lipidated APG8a (arrow, I) were detected in membrane fraction (P) but only non-lipidated LC3 was detected in soluble fraction (S).
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Formalin-fixed and paraffin-embedded human brain tissue reacted with Autophagy LC3 Antibody (APG8a) (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Fluorescent image of U251 cells stained with AP1801a LC3 (APG8A) (N-term) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1801a LC3 (APG8A) (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
Application 
| IHC-P, IF, WB, E |
|---|---|
| Primary Accession | Q9H492 |
| Reactivity | Human, Mouse, Rat |
| Predicted | Bovine, Canine, Chicken |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 14272 Da |
| Antigen Region | 1-30 aa |
| Gene ID | 84557 |
|---|---|
| Other Names | Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, MAP1A/MAP1B LC3 A, Microtubule-associated protein 1 light chain 3 alpha, MAP1LC3A |
| Target/Specificity | This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-term of human LC3 (APG8a). |
| Dilution | IHC-P~~1:50~100 IF~~1:200 WB~~1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | LC3 Antibody (APG8A) (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | MAP1LC3A |
|---|---|
| Function | Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed:20713600, PubMed:24290141). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:20713600). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed:31006537, PubMed:31006538). |
| Cellular Location | Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Endomembrane system; Lipid-anchor. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:Q91VR7}. Note=LC3-II binds to the autophagic membranes. |
| Tissue Location | Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes |
- Primary Antibodies
- Cancer
- Cardiovascular
- Cell Biology
- Metabolism
- Microbiology
- Neuroscience
- Signal Transduction
- Antibody Collections
- Autophagy
- Autophagy
| Author : Liu B1,Ding L1,Zhang L2,Wang S3,Wang Y1,Wang B4,Li L1. Am J Chin Med. 2019 Sep 5:1-14. doi: 10.1142/S0192415X19500721. [Epub ahead of print] Application: WB | 31488033 |
| Author : Leu YS1,Chen YJ2,3,4,Chen CC5,Huang HL6. Am J Chin Med. 2019 Sep 5:1-16. doi: 10.1142/S0192415X19500708. [Epub ahead of print] Application: WB | 31488037 |
| Author : Huang KJ1,Kuo CH2,Chen SH3,Lin CY3,Lee YR3,4. J Cell Mol Med. 2018 Jan 24. doi: 10.1111/jcmm.13474. [Epub ahead of print] Application: WB | 29363886 |
| Author : Paquet C1,2,3,Nicoll JA4,5,Love S6,Mouton-Liger F2,7,Holmes C4,8,Hugon J1,2,3,Boche D4. Brain Pathol. 2017 Oct 13. doi: 10.1111/bpa.12567. [Epub ahead of print] Application: IHC-P | 29027727 |
| Author : Hah YS1,Kim JG2,Cho HY1,Park JS3,Heo EP4,Yoon TJ2. Oncol Lett. 2017 Aug;14(2):1925-1932. doi: 10.3892/ol.2017.6422. Epub 2017 Jun 19. Application: WB | 28781636 |
| Author : Doucet-Beaupré H1, Gilbert C1, Profes MS1, Chabrat A1, Pacelli C2, Giguère N2, Rioux V1, Charest J1, Deng Q3, Laguna A4, Ericson J3, P Proc Natl Acad Sci U S A. 2016 Jul 26;113(30):E4387-96. doi: 10.1073/pnas.1520387113. Epub 2016 Jul 12. Application: WB | 27407143 |
| Author : So EC1,Chen YC2,Wang SC3,Wu CC3,Huang MC3,Lai MS3,Pan BS4,Kang FC5,Huang BM3. Onco Targets Ther. 2016 Apr 27;9:2519-33. doi: 10.2147/OTT.S101671. eCollection 2016. Application: IF | 27175086 |
Provided below are standard protocols that you may find useful for product applications.
Background
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3a is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.