Rapid Transfer Buffer (10X) (Powder)
Référence MBS8325644-5x1L
Conditionnement : 5x1L
Marque : MyBiosource
Rapid Transfer Reagent – Powder
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8325644Quick, efficient transfer of proteins from SDS-PAGE gels to membranes for Western blotting applications More details
Product Overview
Online Datasheet in PDFProduct Name
Rapid Transfer, Reagent
Full Product Name
Rapid Transfer Buffer (10X) (Powder)
Specificity
Fast, efficient transfer in 10 - 30 minutesCompatible with standard wet and semi-dry transfer equipmentMethanol-free, non-hazardous formulationTransfers to PVDF and nitrocellulose from poured or pre-cast gels
Preparation and Storage
Store at 25 degree C for 2 years.
Directions for Use
Stock Solution:1L size: Dissolve the powder in 1L deionized water for Rapid Transfer Buffer (10X).100 ml size: Dissolve the powder in 100 ml deionized water for Rapid Transfer Buffer (10X).Working Solution:Prepare 1 L of Rapid Transfer Buffer (1X) by diluting 100 mL of Rapid Transfer Buffer (10X) with 800 mL of deionized water, then add 100 ml Ethanol.
Product Categories/Family for Rapid Transfer reagent
Reagents
Applications Tested/Suitable for Rapid Transfer reagent
Western Blot (WB)
Application Notes for Rapid Transfer reagent
Wet Transfer Protocol1. Prepare membrane and filter paper for transfer:a. A blotting membrane and 3 pieces of filter paper should be cut to fit dimensions of the gel.Note: PVDF membranes must be pre-wetted according to the manufacturer's instructions in 100% methanol prior to equilibration in transfer buffer.b. Equilibrate the membrane and filter paper in 1X Rapid Transfer Buffer for a minimum of 5 minutes.2. Following protein electrophoresis, assemble the blotting sandwich following the manufacturer's instructions for the transfer apparatus.3. Place the blotting sandwich in a wet transfer tank filled with 1X Rapid Transfer Buffer.4. Transfer constant current 300-400 mA at room temperature.Semi-Dry Transfer Protocol1. Prepare membrane and filter paper for transfer:a. A blotting membrane and 3 pieces of filter paper should be cut to fit dimensions of the gel.Note: PVDF membranes must be pre-wetted according to the manufacturer's instructions in 100% methanol prior to equilibration in transfer buffer.b. Equilibrate the membrane and filter paper in 1X Rapid Transfer Buffer for a minimum of 5 minutes.2. Following electrophoresis, wash the gel for 2 minutes in deionized water.3. Pre-equilibrate the gel in 1X Rapid Transfer Buffer for 5 minutes.4. Assemble the blotting sandwich following the manufacturer's instructions for the semi-dry transfer apparatus.5. Transfer constant current 300-400 mA at room temperature.
Other Notes
Small volumes of Rapid Transfer reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for Rapid Transfer purchase
MBS8325644 is a reagent targeting Rapid Transfer. Also known as Rapid Transfer Buffer (10X) (Powder).