- A 96-well Assay plate --The 96-well plate has been pre-coated with rat IgG.
- Standard (6 x 0.5 ml)--Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
- HRP-conjugated IgG(1 x 6 ml) --Bind to the IgG, and HRP catalyzes the TMB to elicit a chromogenic reaction.
- Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
- Wash Buffer (25x concentrate) 1 x 20 ml --Wash away unbound or free substances.
- TMB Substrate (1x 10 ml) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
Rat Immunoglobulin G,IgG ELISA Kit
Référence CSB-E07981r-96T
Conditionnement : 96T
Marque : Cusabio
| Size | 96T,5×96T,10×96T |
Product Details
Target Name
Immunoglobulin G,IgG
Associated names
N/A
Abbreviation
IgG
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma
Detection Range
31.25 ng/mL-8000 ng/mL
Sensitivity
31.25 ng/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Competitive
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IgG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 100 | ||||
| Range % | 95-104 | |||||
| 1:2000 | Average % | 90 | ||||
| Range % | 86-95 | |||||
| 1:4000 | Average % | 87 | ||||
| Range % | 83-92 | |||||
| 1:8000 | Average % | 101 | ||||
| Range % | 97-106 | |||||
Recovery
| The recovery of rat IgG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 88-95 | ||||
| EDTA plasma (n=4) | 93 | 89-97 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||
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Materials provided
Materials not provided
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm - 570 nm.
- An incubator that can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
The rat immunoglobulin G (IgG) ELISA kit is employed for the quantitative analysis of IgG levels in rat samples. This kit is specifically designed for rat species and is suitable for testing serum and plasma samples. With a detection range of 31.25 ng/mL to 8000 ng/mL and a sensitivity of 31.25 ng/mL, this kit provides both broad detection capabilities and high sensitivity. The kit employs a competitive measurement method, ensuring accurate results. The assay time ranges from 1 to 5 hours, making it efficient for laboratory use. A small sample volume of 50-100 µl is required for analysis, and...
Citations
- S Cai, SK Qi, LN Mao, L Xie, J He,International Journal of Endocrinology,2025
- O Oluyele, MK Oladunmoye,Prospects in Pharmaceutical Sciences,2025
- Impacts of Echinacea purpurea on the immune-reproductive changes associated with the aging process in male rats KH Mohamed,African Journal of Biological Sciences,/
- Y Zhang,Food and chemical toxicology,2023
- ED Kryl'skii,Biochemistry (Moscow),2023
- HTH Ismail,Environmental science and pollution research international,2023
- Y Yan,Advances in clinical and experimental medicine,2023
- S Ghareeb,Toxicology in vitro,2022
- R Toghan,Toxicology,2022
- S Sun,Molecular Nutrition & Food Research,2021
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