RayBio® Rat MCP-1 (CCL2) ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Rat MCP-1 ELISA Kit
Rat MCP-1 ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Rat |
| Sensitivity | 15 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 15 pg/ml - 18000 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: rat CINC-2, CINC-3, CNTF, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MIP-3 alpha, beta-NGF, TIMP-1, TNF-alpha, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Recommended Dilution (Serum/Plasma) | 500 - 10,000 fold |
| Accession Number | P14844 |
| Gene Id | 24770 |
| Gene Symbols | CCL2, SCYA2, MCP1, JE |
| Protein Name / Synonyms | C-C motif chemokine 2 (Immediate-early serum-responsive protein JE) (Monocyte chemoattractant protein 1) (Monocyte chemotactic protein 1) (MCP-1) (Small-inducible cytokine A2) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis, Cancer, Inflammation, Chemokine Biology |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
| Sample type | Cell Culture Supernatants / Plasma / Serum, Cell and Tissue Lysates |
Related Products
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Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Rat MCP-1 ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified MCP-1 (CCL2), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
Excel (Sandwich-ELISA-Analysis-Tool.xlsm)
citations
Olink Proteomics Reveals CCL2 Aggravates Perihematomal Edema After Intracerebral Hemorrhage in High-Altitude Migrants Via CCR2/NF-κB-Mediated Blood-Brain Barrier Disruption
- Species:
- Rat
- Sample Type:
- Whole Blood (peripheral blood); Tissue Lysate / Homogenate (brain tissues)
- Year:
- 2026
- Journal Name:
- CNS Neuroscience & Therapeutics
- PubMed ID:
- 41944579
Immediate icing disrupts the very early MCP-1/CCR2 recruitment window and delays muscle regeneration in rats
- Species:
- Rat
- Sample Type:
- Cell Culture Supernatant (muscle homogenate supernatant)
- Year:
- 2026
- Journal Name:
- American Journal of Physiology-Cell Physiology
- PubMed ID:
- 42284081
Modulation of TNFα-driven neuroinflammation by Gardenin A: insights from in vitro, in vivo, and in silico studies
- Species:
- Rat
- Sample Type:
- Tissue Lysate / Homogenate (brain tissue homogenates)
- Year:
- 2025
- Journal Name:
- Frontiers in Pharmacology
- PubMed ID:
- 41293241
Disease-modifying effects of a glial-targeted inducible nitric oxide synthase inhibitor (1400W) in mixed-sex cohorts of a rat soman (GD) model of epilepsy
- Species:
- Rat
- Sample Type:
- Serum, cerebrospinal fluid (CSF)
- Year:
- 2023
- Journal Name:
- Journal of Neuroinflammation
Protective mechanism of safflower yellow injection on myocardial ischemia-reperfusion injury in rats by activating NLRP3 inflammasome
- Species:
- Rat
- Sample Type:
- Serum (myocardial I/R rats, SYI treatment)
- Year:
- 2025
- Journal Name:
- BMC Complementary Medicine and Therapies
- PubMed ID:
- 39789550
Neat1 lncRNA organizes the inflammatory gene expressions in the dorsal root ganglion in neuropathic pain caused by nerve injury
- Species:
- Rat
- Sample Type:
- spinal cord
- Year:
- 2023
- Journal Name:
- Frontiers in immunology
Alternative Treatments to Exercise for the Attenuation of Disuse-Induced Skeletal Muscle Atrophy in Rats
- Species:
- Rat
- Sample Type:
- Plasma (hindlimb disuse rats)
- Year:
- 2024
- Journal Name:
- Muscles (Basel, Switzerland)
- PubMed ID:
- 40757592
Conditioned Medium from Human Amniotic Membrane-Derived Mesenchymal Stem Cells Modulates Inflammatory and Myofibrotic Factors in Vivo
- Species:
- Rat
- Sample Type:
- Serum (hAM-MSC conditioned medium treatment)
- Year:
- 2024
- Journal Name:
- The journal of Tehran Heart Center
- PubMed ID:
- 40271173
Acetyl-11-Keto-Beta-Boswellic Acid Has Therapeutic Benefits for NAFLD Rat Models That Were Given a High Fructose Diet by Ameliorating Hepatic Inflammation and Lipid Metabolism
- Species:
- Rat
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Inflammation
Mirtazapine, an atypical antidepressant, mitigates lung fibrosis by suppressing NLPR3 inflammasome and fibrosis-related mediators in endotracheal bleomycin rat model
- Species:
- Rat
- Sample Type:
- lung
- Year:
- 2023
- Journal Name:
- Biomedicine & Pharmacotherapy