Rat Monoclonal Anti-GM-CSF (Granulocyte/Macrophage - Colony Stimulating Factor) [Clone SPM469]

Référence NB-36-00088X-P0

Conditionnement : 20ug

Marque : Neo Biotech

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GM-CSF (Granulocyte/Macrophage – Colony Stimulating Factor) Antibody [SPM469]

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Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml

Summary

Granulocyte/macrophage – Colony-stimulating factor (GM-CSF) is a hematopoietic factor that is produced by activated T-cells, B-cells, mast cells, macrophages, fibroblasts, and endothelial cells. In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors.

Product Properties & Targets

Antibody Type
Rat Monoclonals
Host
Rat
Applications
Flow Cytometry, Immunofluorescence
Species Reactivity
Cynomolgus Monkey, Human, Rhesus Monkey
Research Areas
Cardiovascular, Immunology, AKT Signaling, Cytokine Signaling, Hematopoietic Stem Cells, Signal Transduction, Transcription Factors
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Secreted
Gene Name
CSF2
Positive Control
Lymph node and tonsil.
Immunogen
Recombinant human GM-CSF protein
Alternate Names
Granulocyte-macrophage colony-stimulating factor, Colony-stimulating factor, Molgramostin, Sargramostim, Burst Promoting Activity; Colony stimulating factor 2 (granulocyte-macrophage); Eosinophil Colony Stimulating Factor; Granulocyte Macrophage Colony Stimulating Factor; Molgramostin; Pluripoietin Alpha; Sargramostim

Functions

  • Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

Key References

  • Abrams J, et al. 1992. Immunol. Rev. 127:5.
  • Abrams J, et al. 1994. Eosinophils in Allergy and Inflammation. Marcel Dekker New York. p.133.
  • Bacchetta R, et al. 1990. J. Immunol. 144:902.
  • Kita H, et al. 1991. J. Exp. Med. 174:745.
  • Andersson J, et al. 1994. Immunology 83:16.
  • Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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