Recombinant allergen Ara h 2 for Arachis hypogaea (peanut, groundnut)
Référence MBS505124-5x1mg
Conditionnement : 5x1mg
Marque : MyBiosource
Figure 1. SDS-PAGE analysis (15%) of 5 ?l of the recombinant allergen. Purity is >95% as determined by gel electrophoresis. Both bands represent the complete protein, with the higher band containing one Oglycosylation, as determined by MALDI-TOF analysis.
Ara H 2 Recombinant Protein
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS505124RECOMBINANT ALLERGEN: Arachis hypogaea (Peanut, groundnut) Ara h 2 2.02 isoform (Burks et al., 1998).DESCRIPTION: the Arachis hypogaea Ara h 2 is a member of the 2S albumin family and has been prepared as a recombinant allergen fused to a his-tag. It is… More details
Product Overview
Online Datasheet in PDFProduct Name
Ara h 2, Recombinant Protein
Full Product Name
Recombinant allergen Ara h 2 for Arachis hypogaea (peanut, groundnut)
Host
Pichia pastoris
Form/Format
Lyophilized
Concentration
2.32 mg/ml (lot specific)
Preparation and Storage
Protein is shipped with dry ice. Upon arrival, it should be aliquoted to avoid repeated freezing and thawing cycles and stored at -20ºC to -80ºC. In order to defrost the protein, maintain the aliquot at 25ºC without shaking to avoid aggregation.
Molecular Weight
determined by SDS-PAGE, the protein band is between the molecular markers of 35,000 and 25,000 Da, while relative molecular mass calculated from amino acid sequence and without glycosylation is 20,874.76 Da.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Application Notes for Ara h 2 recombinant protein
POSIBLE APPLICATIONS: WB, DB, Indirect ELISA, positive control in direct ELISA, CLIA, lateral-flow. Where this product has not been tested for use in a particular technique, this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates. Recombinant allergens expressed in E. coli do not contain glycosylation, and P. pastoris adds a different glycosylation than the one found in the environment and food allergens. As a result, allergens produced in these expression systems do not need anti-CCD blocker to eliminate the anti-CCD IgE antibodies present in normal human sera. In the specific case when you measure IgG with an allergen produced in P. pastoris, we recommend using our anti-CCD blocker to avoid cross-reaction with antibodies in sera which react with fungal high mannose glycans.
Product References and Citations for Ara h 2 recombinant protein
Burks W, Sampson HA and Bannon GA. Peanut allergens. 1998, Allergy: 725-730.
Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.
Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.
Other Notes
Small volumes of Ara h 2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for Ara h 2 purchase
MBS505124 is a recombinant protein targeting Ara h 2. Also known as Recombinant allergen Ara h 2 for Arachis hypogaea (peanut, groundnut). Host: Pichia pastoris.
Additional Details
NCBI/Uniprot data below describe general gene information for Ara h 2. It may not necessarily be applicable to this product.
Protein Family
Conglutin
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.