Purified recombinant leptin expressed in E.coli. Sterile filtered through a 0.2 micron filter.
Recombinant Human LEPTIN
Recombinant Human LEPTIN
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Product Description
Specifications
| Size | 1 mg |
| Species | Human |
| Protein Name / Synonyms | LEPTIN, |
| Purity | >98% by SDS PAGE and HPLC analysis. |
| Molecular Weight (kDa) | 16.0kD (147 amino acid residues). |
| Format | Purified recombinant protein - lyophilized |
| Formulation | Lyophilized with 0.1% TFA |
| Concentration (lot specific) | ~1.0 mg/ml after reconstitution |
| Preservative | None present |
| Activity | 129-20189 is biologically active in the ob/ob mouse obesity model. The ob/ob mice were treated via intraperitoneal injection once daily at a dose of 5 µ,g Leptin/gm of body weight for 7 days. Significant effects on body weight, food con |
| Recommended Applications | ELISA (0.2-0.4ng/well), Functional Assays, Western Blotting (1.5-3.0ng/lane), ELISA (0.2-0.4ng/well), Functional Assays, Western Blotting (1.5-3.0ng/lane) |
| Endotoxin Level | <1EU/ug |
| Research Area | Energy Metabolism, Obesity & Metabolic Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Introduction
Leptin is a major adipose-derived hormone and member of the leptin family, which plays a key role in appetite control and the regulation of energy expenditure.
Secreted leptin associates with proteins within the bloodstream and is transported to the brain, where it inhibits or up-regulates the activity of neuropeptides such as neuropeptide Y (NPY) and alpha-melanocyte-stimulating hormone respectively. Leptin is a recognised marker of obesity, with a correlation between circulating levels of leptin and body fat mass, and defects in leptin production result in severe obesity. Regulators of leptin expression include insulin, cortisol, cAMP and thiazolidinediones.
Secreted leptin associates with proteins within the bloodstream and is transported to the brain, where it inhibits or up-regulates the activity of neuropeptides such as neuropeptide Y (NPY) and alpha-melanocyte-stimulating hormone respectively. Leptin is a recognised marker of obesity, with a correlation between circulating levels of leptin and body fat mass, and defects in leptin production result in severe obesity. Regulators of leptin expression include insulin, cortisol, cAMP and thiazolidinediones.
Specifications
Size
1 mg
Stability & Storage
Storage/Stability
Prior to reconstitution store at +4°C.
After reconstitution store at -20°C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. This protein may be stored for3 months from date of reconstitution.
After reconstitution store at -20°C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. This protein may be stored for3 months from date of reconstitution.