RH FGF-8
Référence 800-03-100UG
Conditionnement : 100UG
Marque : FUJIFILM
Recombinant Human / Mouse FGF-8
Fibroblast growth factor 8 (FGF-8) is a critical mitogenic factor that is required for normal development of the eye, ear, brain, and limb. FGF-8 functions broadly to promote cell proliferation, differentiation, and migration. Overexpression of FGF-8 increases tumor growth and angiogenesis. Human and mouse FGF-8 proteins show 100% homology.
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Instructions
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
Technical Specifications
| Alternate Names | Androgen-induced growth factor, AIGF, Heparin-binding growth factor 8, HBGF-8, FGF-8b isoform |
| Accession Number | P55075/P37237 |
| Source | Genetically modified E.coli |
| Predicted Molecular Mass | Monomer, 22.5 kDa (194 aa) |
| AA Sequence | MQVTVQSSPN FTQHVREQSL VTDQLSRRLI RTYQLYSRTS GKHVQVLANK RINAMAEDGD PFAKLIVETD TFGSRVRVRGAETGLYICMN KKGKLIAKSN GKGKDCVFTE IVLENNYTAL QNAKYEGWYM AFTRKGRPRK GSKTRQHQRE VHFMKRLPRG HHTTEQSLRFEFLNYPPFTR SLRGSQRTWA PEPR |
| Formulation | Lyophilized from a 0.2 µm filtered solution containing 0.1% Trifluoroacetic Acid (TFA) |
| Product Reconstitution | Sterile water at 0.1 mg/mL |
| Country of Origin | USA |
| Specification* | Method of Determination | Acceptance Criteria |
| Purity | Reducing and Non-Reducing SDS PAGE | ≥ 95% |
| Endotoxin | Kinetic LAL | ≤ 0.1 EU/µg |
| Biological Activity (ED50) | NR6R-3T3 cell proliferation | ≤ 150 ng/mL |
*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.