RH FGF-BASIC 147 (AF)
Référence 100-28AF-50UG
Conditionnement : 50UG
Marque : FUJIFILM
Recombinant Human FGF-basic 147 / FGF-2
Basic fibroblast growth factor (FGF-basic), also known as FGF-2, is expressed by endothelial cells and is a mediator of angiogenesis. FGF-basic also has cardioprotective functions during heart injury. FGF-basic is a critical component for embryonic stem cell culture systems and is necessary for maintaining cells in an undifferentiated state. Degradation of the full length FGF-basic N-terminus results in a truncated FGF-basic 147aa protein, when the protein is isolated from biological sources. The N-terminus extensions influence the localization of FGF-basic within the cell, but do not affect the biological activity of FGF-basic. Therefore, there are no detectable differences in biological activity between the full length FGF-basic 154aa and the truncated FGF-basic 147aa recombinant proteins.
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SDS-PAGE Showing a Single Prominent Band at 16.5 kDa
Figure 1. rh FGF-basic 147 (1μg) was resolved with SDS-PAGE under reducing (R) conditions and visualized by Instant Blue staining showing a single prominent band at 16.5 kDa (R).
Cell-Based Proliferation Assay
Figure 2. Recombinant Human FGF-basic 147 / FGF-2 induced proliferation in NR6R/3T3 cells.
Instructions
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. If a precipitate is observed, centrifuge the solution thoroughly and use only the soluble fraction (removing it from the precipitate). A 10% overfill has been added to compensate for any loss of protein in the precipitate. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
Technical Specifications
| Alternate Names | FGF2, HBGF-2, Prostatropin |
| Accession Number | P09038 |
| Source | Genetically modified E.coli |
| Predicted Molecular Mass | Monomer, 16.5 kDa (147 aa) |
| AA Sequence | MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS |
| Formulation | Lyophilized from a 0.2 µm filtered solution containing 10 mM sodium phosphate, 75 mM sodium chloride, pH 7.5 |
| Product Reconstitution | Sterile water at 0.1 mg/mL |
| Country of Origin | USA |
| Specification* | Method of Determination | Acceptance Criteria |
| Purity | Reducing and Non-Reducing SDS PAGE | ≥ 95% |
| Endotoxin | Kinetic LAL | ≤ 0.1 EU/µg |
| Biological Activity (ED50) | NR6R-3T3 cell proliferation | ≤ 5 ng/mL; ≥ 2.0 x 105 units/mg |
*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.