RH GDNF
Référence 100-02-100UG
Conditionnement : 100UG
Marque : FUJIFILM
Recombinant Human GDNF
Glial cell-derived neurotrophic factor (GDNF) is a neurotrophic factor that signals through a multicomponent receptor system to activate receptor tyrosine kinase RET signaling. GDNF promotes dopamine uptake, prevents motor neuron apoptosis, and supports the survival and differentiation of neurons.
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SDS-PAGE Showing a Single Prominent Band at 30.4 kDa
Figure 1. rh GDNF (1μg) was resolved with SDS-PAGE under non-reducing (NR) conditions and visualized by Instant Blue staining showing a single prominent 30.4 kDa (NR)
Cell-Based Proliferation Assay
Figure 2. Recombinant Human GDNF induced proliferation in C6 Glioma cells.
Instructions
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
Technical Specifications
| Alternate Names | ATF-1 |
| Accession Number | P39905 |
| Source | Genetically modified E.coli |
| Predicted Molecular Mass | Dimer, 15.2/30.4 kDa (135/270 aa) |
| AA Sequence | MSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI |
| Formulation | Lyophilized from a 0.2 µm filtered solution containing 10 mM sodium citrate, 100 mM sodium chloride, pH 4.0 |
| Product Reconstitution | Sterile water at 0.1 mg/mL |
| Country of Origin | USA |
| Specification* | Method of Determination | Acceptance Criteria |
| Purity | Reducing and Non-Reducing SDS PAGE | ≥ 95% |
| Endotoxin | Kinetic LAL | ≤ 0.1 EU/µg |
| Biological Activity (ED50) | C6 proliferation | ≤ 3 µg/mL; ≥ 3.3 x 102 units/mg |
*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.