RH IFN-A 2A
Référence 100-54-1MG
Conditionnement : 1MG
Marque : FUJIFILM
Recombinant Human IFN-α 2a
Interferon-alpha 2a (IFN-α 2a) is a type I interferon made by leukocytes during viral infection. The JAK-STAT pathway mediates the antiviral and anti-cell proliferation activities of IFN-α 2a. IFN-α proteins are widely used as standard treatments during antiviral and antineoplastic therapies. The IFN-α 2a variant differs from IFN-α 2b by one amino acid.
Also available as a GMP product: CTGrade GMP rh IFN-alpha 2A
Product images on the website are not representative of actual product, including, but not limited to differences in cap color, tube type, or label format.
Instructions
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
Technical Specifications
| Alternate Names | Type I Interferon, Alpha 2 |
| Accession Number | P01563 |
| Source | Genetically modified E.coli |
| Predicted Molecular Mass | Monomer, 19.4 kDa (166 aa) |
| AA Sequence | MCDLPQTHSL GSRRTLMLLA QMRKISLFSC LKDRHDFGFP QEEFGNQFQK AETIPVLHEM IQQIFNLFST KDSSAAWDET LLDKFYTELY QQLNDLEACV IQGVGVTETP LMKEDSILAV RKYFQRITLY LKEKKYSPCA WEVVRAEIMR SFSLSTNLQE SLRSKE |
| Formulation | Lyophilized from a 0.2 µm filtered solution containing 10 mM sodium phosphate and 140 mM NaCl, pH 7.4 |
| Product Reconstitution | Sterile water at 0.1 mg/mL |
| Country of Origin | USA |
| Specification* | Method of Determination | Acceptance Criteria |
| Purity | Reducing and Non-Reducing SDS PAGE | ≥ 98% |
| Endotoxin | Kinetic LAL | ≤ 0.1 EU/µg |
| Biological Activity (ED50) | Induction of cytotoxicity of TF-1 cells by rh IFN-α 2a | ≤ 0.3 ng/mL; ≥ 3.3 x 106 units/mg |
*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.