RH IGF-I
Référence 100-34-20UG
Conditionnement : 20UG
Marque : FUJIFILM
Recombinant Human IGF-I
Insulin-like growth factor I (IGF-I) is a growth factor that is produced by the liver. IGF-I production is stimulated by growth hormone (GH). IGF-I binds the insulin-like growth factor I receptor (IGF-I receptor) and the insulin receptor to stimulate systemic body growth. IGF-I is one of the most potent activators of the AKT signaling pathway, which stimulates cell proliferation and inhibits programmed cell death.
Also available as a GMP product: CTGrade GMP rh IGF-1
Product images on the website are not representative of actual product, including, but not limited to differences in cap color, tube type, or label format.
SDS-PAGE Showing a Single Prominent Band at 7.7 kDa
Figure 1. rh IGF1 (1μg) was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by Instant Blue staining showing a single prominent band at 7.7 kDa.
Cell-Based Proliferation Assay
Figure 2. Recombinant Human IGF-I induced proliferation of MCF-7 cells.
Instructions
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
Technical Specifications
| Alternate Names | Somatomedin C, mechano growth factor, IGF-IA |
| Accession Number | P05019 |
| Source | Genetically modified E.coli |
| Predicted Molecular Mass | Monomer, 7.7 kDa (70 aa) |
| AA Sequence | GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA |
| Formulation | Lyophilized from a 0.2 µm filtered solution containing 0.1% Trifluoroacetic Acid (TFA) |
| Product Reconstitution | Sterile water at 0.1 mg/mL |
| Country of Origin | USA |
| Specification* | Method of Determination | Acceptance Criteria |
| Purity | Reducing and Non-Reducing SDS PAGE | ≥ 95% |
| Endotoxin | Kinetic LAL | ≤ 0.1 EU/µg |
| Biological Activity (ED50) | MCF-7 cell proliferation | ≤ 1.5 ng/mL; ≥ 6.7 x 105 units/mg |
*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.