RH IL-1B (AF)
Référence 100-167AF-10UG
Conditionnement : 10UG
Marque : FUJIFILM
Interleukin 1 beta (IL-1β) is a pro-inflammatory cytokine that is produced by monocytes, macrophages, and dendritic cells (DCs). IL-1β signals through the IL-1 receptor, type 1 (IL-1R1) to activate the myeloid differentiation primary response 88 (MYD88) signaling pathway, which contains the cytoplasmic Toll/IL-1 receptor (TIR) domain adapter. IL-1β and the independently regulated IL-1α protein have overlapping pro-inflammatory activities to induce adhesion molecule expression on epithelial cells, control fever induction, initiate rheumatoid arthritis, and promote septic shock.
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SDS-PAGE Showing a Single Prominent Band at 17.5 kDa
Figure 1. rh IL-1β (1μg) was resolved withSDS-PAGE under non-reducing(NR) and reducing (R) conditionsand visualized by Instant Bluestaining showing a single prominent band at 17.5 kDa.
Cell based proliferation assay
Figure 2. Recombinant Human IL 1 Beta induced proliferation of D10G4.1 cells
Instructions
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
Technical Specifications
| Alternate Names | Catabolin, LAF, EP, LEM, MCF |
| Accession Number | P01584 |
| Source | Genetically modified E.coli |
| Predicted Molecular Mass | Monomer, 17.5 kDa (154 aa) |
| AA Sequence | MAPVRSLNCT LRDSQQKSLV MSGPYELKAL HLQGQDMEQQ VVFSMSFVQG EESNDKIPVA LGLKEKNLYL SCVLKDDKPT LQLESVDPKN YPKKKMEKRF VFNKIEINNK LEFESAQFPN WYISTSQAEN MPVFLGGTKG GQDITDFTMQ FVSS |
| Formulation | Lyophilized from a 0.2 µm filtered solution containing 10 mM sodium phosphate, pH 7.5 |
| Product Reconstitution | Sterile water at 0.1 mg/mL |
| Country of Origin | USA |
| Specification* | Method of Determination | Acceptance Criteria |
| Purity | Reducing and Non-Reducing SDS PAGE | ≥ 95% |
| Endotoxin | Kinetic LAL | ≤ 1 EU/µg |
| Biological Activity (ED50) | D10S cell proliferation | ≤ 0.012 ng/mL; ≥ 8.3 x 107 units/mg |
*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.