RH PDGF-BB
Référence 100-18-10UG
Conditionnement : 10UG
Marque : FUJIFILM
Recombinant Human PDGF-BB
Platelet-derived growth factor (PDGF) is an important regulator of cell growth, proliferation, and angiogenesis. PDGF synthesis is induced by IL-1, IL-6, TNF-α, TGF-β, and EGF signaling. PDGF functions as a mitogenic growth hormone on cells of mesenchymal lineage, such as smooth muscle and glial cells. PDGF is also stored in the alpha-granules of platelets and is released upon adherence to traumatized tissues. PDGF is a dimeric glycoprotein formed by two A chains (AA), two B chains (BB), or as a heterodimer with an A and a B chain (AB). The PDGF dimer binds the cell surface receptor tyrosine kinases PDGFR-α and PDGFR-β.
Also available as a GMP product: CTGrade GMP rh PDGF-BB
Product images on the website are not representative of actual product, including, but not limited to differences in cap color, tube type, or label format.
SDS-PAGE Showing a Prominent Band at 12.4 kDa and 24.9 kDa
Figure 1. rh PDGF-BB (1 μg) was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by InstantBlue staining showing a single prominent band at 12.4 kDa (R) and 24.9 kDa (NR).*
*Predicted MW is 12.4 kDa and 24.9 kDa, but runs on SDS-PAGE at ~15 kDa (R) and ~30 kDa (NR)
Cell-Based Proliferation Assay
Figure 2. Recombinant Human PDGF-BB induced proliferation in NR6R/3T3 cells.
Instructions
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
Technical Specifications
| Alternate Names | GDGF, ODGF |
| Accession Number | P01127 |
| Source | Genetically modified E.coli |
| Predicted Molecular Mass | Dimer, 12.4/24.9 kDa (110/220 aa) |
| AA Sequence | MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETVAAARPVT |
| Formulation | Lyophilized from a 0.2 µm filtered solution containing 10 mM sodium phosphate, pH 7.5 |
| Product Reconstitution | Sterile water at 0.1 mg/mL |
| Country of Origin | USA |
| Specification* | Method of Determination | Acceptance Criteria |
| Purity | Reducing and Non-Reducing SDS PAGE | ≥ 95% |
| Endotoxin | Kinetic LAL | ≤ 0.1 EU/µg |
| Biological Activity (ED50) | NR6R-3T3 cell proliferation | ≤ 20 ng/mL; ≥ 5.0 x 104 units/mg |
*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.