RH TNF-A (AF)
Référence 100-111AF-100UG
Conditionnement : 100UG
Marque : FUJIFILM
Recombinant Human TNF-α
Tumor necrosis factor alpha (TNF-α) is an inflammatory cytokine secreted by macrophages, monocytes, neutrophils, T cells, and NK-cells following stimulation by bacterial lipopolysaccharide (LPS). TNF-α signal activation occurs through two receptors, TNFR1 and TNFR2. TNFR1 is expressed on most cell types, unlike TNFR2, which is expressed mainly on immune cells. TNF-α functions to stimulate phagocytosis in macrophages, chemoattract neutrophils, increase insulin resistance, and induce fever.
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SDS-PAGE Showing a Single Prominent Band at 17.5 kDa
Figure 1. rh TNF-α (1μg) was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by Instant Blue staining showing a single prominent band at 17.5kDa (+)
Cell based cytotoxicity assay
Figure 2. Recombinant Human TNF-α induced cytolysis of mouse L929 cells in the presence of Actinomycin D
Instructions
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution.
Technical Specifications
| Alternate Names | TNFSF2, Cachectin, DIF, Necrosin, Cytotoxin, Cachexin, TNF |
| Accession Number | P01375 |
| Source | Genetically modified E.coli |
| Predicted Molecular Mass | Monomer, 17.5 kDa (158 aa) |
| AA Sequence | MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL |
| Formulation | Lyophilized from a 0.2 µm filtered solution containing 10 mM sodium phosphate, pH 7.5 |
| Product Reconstitution | Sterile water at 0.1 mg/mL |
| Country of Origin | USA |
| Specification* | Method of Determination | Acceptance Criteria |
| Purity | Reducing and Non-Reducing SDS PAGE | ≥ 95% |
| Endotoxin | Kinetic LAL | ≤ 0.1 EU/µg |
| Biological Activity (ED50) | Cytolysis of mouse L929 cells in the presence of Actinomycin D | ≤ 2 ng/mL; ≥ 5.0 x 105 units/mg |
*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.