RM GM-CSF
Référence 200-15-5UG
Conditionnement : 5UG
Marque : FUJIFILM
Recombinant Mouse GM-CSF
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is hematopoietic growth factor produced by endothelial cells, monocytes, fibroblasts, and T cells. GM-CSF stimulates the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells. GM-CSF promotes immune system development and regulates neutrophil function during infection. Human and mouse GM-CSF show no cross-reactivity.
Product images on the website are not representative of actual product, including, but not limited to differences in cap color, tube type, or label format.
SDS-PAGE Showing a Single Prominent Band at 14.3 kDa
Figure 1. rm GM-CSF(1μg) was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by Instant Blue staining showing a single prominent band at 14.3 kDa
Cell-Based Proliferation Assay
Figure 2. Recombinant Mouse GM-CSF induced proliferation in FDCP-1 cells.
Instructions
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
Technical Specifications
| Alternate Names | CSF-2, Pluripoietin-α, MGI-1GM |
| Accession Number | P01587 |
| Source | Genetically modified E.coli |
| Predicted Molecular Mass | Monomer, 14.3 kDa (125 aa) |
| AA Sequence | MAPTRSPITV TRPWKHVEAI KEALNLLDDM PVTLNEEVEV VSNEFSFKKL TCVQTRLKIF EQGLRGNFTK LKGALNMTAS YYQTYCPPTP ETDCETQVTT YADFIDSLKT FLTDIPFECK KPVQK |
| Formulation | Lyophilized from a 0.2 µm filtered solution containing 10 mM acetic acid |
| Product Reconstitution | Sterile water at 0.1 mg/mL |
| Country of Origin | USA |
| Specification* | Method of Determination | Acceptance Criteria |
| Purity | Reducing and Non-Reducing SDS PAGE | ≥ 95% |
| Endotoxin | Kinetic LAL | ≤ 0.1 EU/µg |
| Biological Activity (ED50) | FDCP-1 cell proliferation | ≤ 0.05 ng/mL |
*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.