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> Seocalcitol [134404-52-7]

Seocalcitol [134404-52-7]

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Référence HY-32341-1mg

Conditionnement : 1mg

Marque : MedChemExpress


Description

Seocalcitol is a vitamin D analog, binds vitamin D receptor protein from human osteosarcoma MG-63 cells with Kd of 0.27 nM.

IC50 & Target

Kd: 0.27 nM (vitamin D receptor)[1]
Cellular Effect
Cell Line Type Value Description References
U-937 IC50
3.0 x 10-11M
Compound: EB 1089
Compound was tested in vitro to inhibit proliferation of U 937 human histiolytic lymphoma cells
Compound was tested in vitro to inhibit proliferation of U 937 human histiolytic lymphoma cells
10.1016/S0960-894X(00)80126-2
Vero CC50
11 μM
Compound: EB1089
Cytotoxicity against African green monkey Vero cells infected with ZIKV HPF2013 pretreated with virus for 1 hr followed by infection of cells with compound treated-virus for 30 hrs by MTS assay
Cytotoxicity against African green monkey Vero cells infected with ZIKV HPF2013 pretreated with virus for 1 hr followed by infection of cells with compound treated-virus for 30 hrs by MTS assay
[PMID: 36623328]
Vero CC50
11 μM
Compound: EB1089
Cytotoxicity against African green monkey Vero cells infected with ZIKV MR766 pretreated with virus for 1 hr followed by infection of cells with compound treated-virus for 30 hrs by MTS assay
Cytotoxicity against African green monkey Vero cells infected with ZIKV MR766 pretreated with virus for 1 hr followed by infection of cells with compound treated-virus for 30 hrs by MTS assay
[PMID: 36623328]
Vero CC50
11.9 μM
Compound: EB1089
Cytotoxicity against African green monkey Vero cells infected with USUV 3345 isolate Arb276 pretreated with virus for 1 hr followed by infection of cells with compound treated-virus for 20 hrs by MTS assay
Cytotoxicity against African green monkey Vero cells infected with USUV 3345 isolate Arb276 pretreated with virus for 1 hr followed by infection of cells with compound treated-virus for 20 hrs by MTS assay
[PMID: 36623328]
In Vitro

Seocalcitol (EB 1089) is a stimulators of osteoclast recruitment in murine bone marrow cultures, with EC50 at 0.1 nM. Seocalcitol stimulates bone resorption with an estimated EC50 at 0.03 nM[1]. Seocalcitol (EB 1089) elicites a dose-dependent induction of 24-hydroxylase mRNA in the kidney (EC50=0.4±0.13). In the kidney, Kd values for Seocalcitol is 0.48±0.04 nM. However, in the intestine, the Kd for Seocalcitol is 1.43±0.19 nM)[2]. Seocalcitol (0.1-10 nM) induces cell differentiation in a dosedependent manner. A higher differentiating activity is observed for 1 nM Seocalcitol (EB 1089) than for 1 nM VD3.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Seocalcitol Related Antibodies

Solvant et solubilité
In Vitro: 

DMSO : ≥ 50 mg/mL (109.97 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1993 mL 10.9967 mL 21.9935 mL
5 mM 0.4399 mL 2.1993 mL 4.3987 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (5.50 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (5.50 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Pureté et documentation
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