Conditionnement : 1mLx10mM(inDMSO)
Synonyms: 5-hydroxytryptamine, 5-HT
| Description | Serotonin (5-hydroxytryptamine) is a monoamine neurotransmitter in the CNS and an endogenous 5-HT receptor agonist. Serotonin regulates the nervous system, digestive system and cardiovascular system. |
| Targets & IC50 | 5-HT1A:117 nM (EC50) |
| In vitro | METHODS: Mouse B16 melanoma cells were treated with Serotonin for 2 days to detect the anti-melanin production activity. RESULTS: Serotonin is slightly active or inactive in the inhibitory medium dimming, with IC50> 30 μM. [1] |
| In vivo | METHODS: To study the pain hypersensitivity of Serotonin, Serotonin (10 uM; 20 μl was intradermal injected into mice, and the mice were tested at 15, 30, 45, 60, 90, 120, 180 and 240 minutes. RESULTS: Compared with the control group receiving saline treatment, Serotonin produced a strong hypersensitivity reaction (p<0.001). [2] METHODS: To study the role of IL-13-mediated 5-HT signaling in the pathogenesis of colitis, Serotonin (100 mg/kg) was subcutaneously injected into male IL-13-deficient mice 3 days before induction of DSS colitis, twice a day for 8 consecutive days. RESULTS: After inducing DSS colitis, the content of 5-HT in the colon of IL-13-/- mice that received Serotonin increased significantly. [3] |
| Synonyms | 5-hydroxytryptamine, 5-HT |
| Molecular Weight | 176.22 |
| Formula | C10H12N2O |
| Cas No. | 50-67-9 |
| Smiles | NCCc1c[nH]c2ccc(O)cc12 |
| Relative Density. | 1.1102 g/cm3 (Estimated) |
| Storage | Store at low temperature,Keep away from moisture,Keep away from direct sunlight Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. | |||||||||||||||||||||||||||||||||||
| Solubility Information | DMSO: 255 mg/mL (1447.05 mM), Sonication is recommended.  | |||||||||||||||||||||||||||||||||||
| In Vivo Formulation | 10% DMSO+40% PEG300+5% Tween-80+45% Saline: 3.3 mg/mL (18.73 mM), Sonication is recommended. Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions. | |||||||||||||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||||||||||||
DMSO
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density. | ||||||||||||||||||||||||||||||||||||
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
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