Biological Activity

SN-38 is an active metabolite of CPT-11, inhibits DNA topoisomerase I, DNA synthesis and causes frequent DNA single-strand breaks.

Product Citations

• 

  BMC Biol. 2021 May 20;19(1):108.

  Very long intergenic non-coding (vlinc) RNAs directly regulate multiple genes in cis and trans
  SN-38 purchased from AbMole

• 

  Nat Commun. 2019 Dec 20;10(1):5799.

  Novel approach reveals genomic landscapes of single-strand DNA breaks with nucleotide resolution in human cells.
  SN-38 purchased from AbMole

Customer Product Validations & Biological Datas

	Source	Customer experimental result (2018).
	Method	Cell viability assay
	Cell Lines	MDA-MB-231 cells
	Concentrations	32nM
	Incubation Time	4 days
	Results	MDA-MB-231 cells were treated with 32nM SN-38 for 4 days and then incubated for 4 days. Most of the MDA-MB-231 cells were transformed into senescent cells.

	Source	J Immunol Res (2018). Figure 1. SN-38
	Method	flow cytometry
	Cell Lines	NB cell lines
	Concentrations	1 nM
	Incubation Time	24 hours
	Results	Cisplatin at 2 μM, etoposide at 0.1 μM, irinotecan at 1 nM, and topotecan at 10nM induced apoptosis of all NB cell lines after 48 hours of treatment, with the exception of SK-N-AS treated with the first three drugs and SK-N-BE(2)c treated with all four drugs

Protocol (for reference only)

Cell Experiment
Cell lines	A-172, U-87, and LA-567
Preparation method	MTT assay
Concentrations	0 -1000 nM
Incubation time	48 h

Animal Experiment
Animal models	Nontumor-bearing mice
Formulation	dissolved in DMSO (1 mg/mL)
Dosages	1 mg/kg
Administration	i.v.

Chemical Information

Molecular Weight	392.4
Formula	C22H20N2O5
CAS Number	86639-52-3
Solubility (25°C)	DMSO 21 mg/mL
Storage	Powder          -20°C   3 years ;  4°C   2 years In solvent       -80°C   6 months ;  -20°C   1 month

References

[1] Stewart CF, et al. Cancer Chemother Pharmacol. Disposition of irinotecan and SN-38 following oral and intravenous irinotecan dosing in mice.