General Information
| Short Description | This SP Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in all serum, plasma and other biological fluids. |
| Applications | ELISA |
| Reactivity | All |
| Sensitivity | 46.875pg/mL |
| Detection Limit | 78.125~5000pg/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 2.5h |
| Detection | Colormetric |
Target Information
| Specificity | This kit recognizes SP in samples. No significant cross-reactivity or interference between SP and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Background | This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with SP. During the reaction, SP in the sample or standard competes with a fixed amount of SP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to SP. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SP in the samples is then determined by comparing the OD of the samples to the standard curve. |
Information sourced from Uniprot.org